| Summary: | Objective: To study the effect of smoking on EA and NOS expression as well as NO and ET-1
content in gingival tissue of patients with chronic periodontitis. Methods: Patients diagnosed
with periodontitis in our hospital between May 2013 and March 2016 were selected to screen
72 cases of smokers and 80 cases of non-smokers who were enrolled in smoking group and
non-smoking group respectively, periodontal tissue was collected to detect the expression of
EA, NOS and NLRP3 inflammasome, and gingival crevicular fluid was collected to detect
the content of ET-1, NO, inflammatory factors and MMPs. Results: EA expression and ET-1
content in gingival tissue of smoking group were significantly higher than those of nonsmoking
group while NOS expression and NO content in gingival tissue were significantly
lower than those of non-smoking group; NLRP3, ASC, Caspase-1, IL-1β and IL-18
expression in gingival tissue of smoking group were significantly higher than those of nonsmoking
group, and IL-1β, IL-18, TNF-α, IFN-γ, MMP1, MMP8 and MMP13 content in
gingival crevicular fluid were significantly higher than those of non-smoking group; NLRP3,
ASC, Caspase-1, IL-1β and IL-18 expression as well as IL-1β, IL-18, TNF-α, IFN-γ,
MMP1, MMP8 and MMP13 content were positively correlated with EA and ET-1, and
negatively correlated with NOS and NO. Conclusion: Smoking can cause increased EA and
ET-1 as well as decreased NOS and NO in gingival tissue of patients with chronic periodontitis,
thus adjusting the expression of NLRP3 inflammasome and MMPs to periodontal tissue
inflammation and structure damage.
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