A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies

• Main Authors: P. Ó Broin, N. Hayde, Y. Bao, B. Ye, R.B. Calder, G. de Boccardo, M. Lubetzky, M. Ajaimy, J. Pullman, A. Colovai, E. Akalin, A. Golden
• Format: Article
• Language: English
• Published: Elsevier 2014-12-01
• Series: Genomics Data
• Subjects: Kidney; Transplant; Antibody-mediated rejection; Donor-specific antibodies; Gene expression
• Online Access: http://www.sciencedirect.com/science/article/pii/S2213596014000920

Affymetrix Human Gene 1.0-ST arrays were used to assess the gene expression profiles of kidney transplant patients who presented with donor-specific antibodies (DSAs) but showed normal biopsy histopathology and did not develop antibody-mediated rejection (AMR). Biopsy and whole-blood profiles for these DSA-positive, AMR-negative (DSA+/AMR−) patients were compared to both DSA-positive, AMR-positive (DSA+/AMR+) patients as well as DSA-negative (DSA−) controls. While individual gene expression changes across sample groups were relatively subtle, gene-set enrichment analysis using previously identified pathogenesis-based transcripts (PBTs) identified a clear molecular signature involving increased rejection-associated transcripts in AMR− patients. Results from this study have been published in Kidney International (Hayde et al., 2014 [1]) and the associated data have been deposited in the GEO archive and are accessible via the following link: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50084