Concentración mínima inhibitoria de Kanamicina para callos de cuatro variedades colombiana de arroz

• Main Authors: Alejandro Chaparro Giraldo, Cristina Díaz-Granados
• Format: Article
• Language: Spanish
• Published: Universidad Nacional de Colombia 2012-11-01
• Series: Revista Colombiana de Biotecnología
• Subjects: Variedades colombianas de arroz, callogénesis, regeneración, agente de selección.
• Online Access: http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/24996
• ISSN: 0123-3475; 1909-8758

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mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Calibri","sans-serif"; mso-bidi-font-family:"Times New Roman";} --> <!--[endif] --> <p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: justify; line-height: normal;"><span style="font-size: 12pt; font-family: ">La kanamicina (Km) es un antibiótico que se ha utilizado en la producción de plantas transgénicas de arroz como agente selectivo. En este trabajo, se evaluó la sensibilidad al antibiótico para callos derivados de semillas sexuales en cuatro variedades colombianas de arroz (FEDEARROZ 2000, FEDEARROZ 50, FEDEARROZ 369 y CICA 8). Inicialmente, se indujo la formación de callo <span> </span>en un medio específico a partir de semilla madura, obteniéndose porcentajes de callogénesis mayores al 90%. Posteriormente los callos son llevados a un medio de regeneración, en el que se presentan variaciones importantes en el porcentaje de regeneración, entre 62% (F 2000) y 11% (F 369). Se ensayaron 7 concentraciones diferentes de kanamicina, adicionados al medio de regeneración: 0<span>  </span>mg/l, 100 mg/l, 200 mg/l, 300 mg/l, 400 mg/l, 500 mg/l y 600 mg/l.<span>  </span>Se observó respuesta diferencial al antibiótico. Para F2000 y F369 a partir de <span> </span>500 mg/L no registraron regenerantes, mientras para F50 y CICA 8 fue desde 300 mg/L. En todos los casos se observaron regenerantes albinos. </span></p>