A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins

A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins

We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~...

Full description

Bibliographic Details
Main Authors: Roberto Fabian, Santosh Gaire, Christopher Tyson, Raghabendra Adhikari, Ian Pegg, Abhijit Sarkar
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Molecules
Subjects:
single molecule micromanipulation techniques
horizontal magnetic tweezers
DNA-histone interactions
nucleosomes
Online Access:https://www.mdpi.com/1420-3049/26/16/4781
id doaj-466de3cd36e14ff89bef7dd0b84e00c8
record_format Article
spelling doaj-466de3cd36e14ff89bef7dd0b84e00c82021-08-26T14:07:06ZengMDPI AGMolecules1420-30492021-08-01264781478110.3390/molecules26164781A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding ProteinsRoberto Fabian0Santosh Gaire1Christopher Tyson2Raghabendra Adhikari3Ian Pegg4Abhijit Sarkar5Department of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USADepartment of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USABiomedical Engineering Department and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USADepartment of Biology, The Catholic University of America, Washington, DC 20064, USADepartment of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USADepartment of Physics and Vitreous State Laboratory, The Catholic University of America, Washington, DC 20064, USAWe report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~74 pN. During the assembly events, we observed the length of the DNA decrease in approximate integer multiples of ~50 nm, suggesting the binding of the histone octamers to the DNA tether. During the mechanically induced disassembly events, we observed disruption lengths in approximate integer multiples of ~50 nm, suggesting the unbinding of one or more octamers from the DNA tether. We also observed histone octamer unbinding events at forces as low as ~2 pN. Our horizontal magnetic tweezers yielded high-resolution, low-noise data on force-mediated DNA-core histone assembly and disassembly processes.https://www.mdpi.com/1420-3049/26/16/4781single molecule micromanipulation techniqueshorizontal magnetic tweezersDNA-histone interactionsnucleosomes
collection DOAJ
language English
format Article
sources DOAJ
author Roberto Fabian
Santosh Gaire
Christopher Tyson
Raghabendra Adhikari
Ian Pegg
Abhijit Sarkar
spellingShingle Roberto Fabian
Santosh Gaire
Christopher Tyson
Raghabendra Adhikari
Ian Pegg
Abhijit Sarkar
A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
Molecules
single molecule micromanipulation techniques
horizontal magnetic tweezers
DNA-histone interactions
nucleosomes
author_facet Roberto Fabian
Santosh Gaire
Christopher Tyson
Raghabendra Adhikari
Ian Pegg
Abhijit Sarkar
author_sort Roberto Fabian
title A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
title_short A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
title_full A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
title_fullStr A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
title_full_unstemmed A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
title_sort horizontal magnetic tweezers for studying single dna molecules and dna-binding proteins
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2021-08-01
description We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~74 pN. During the assembly events, we observed the length of the DNA decrease in approximate integer multiples of ~50 nm, suggesting the binding of the histone octamers to the DNA tether. During the mechanically induced disassembly events, we observed disruption lengths in approximate integer multiples of ~50 nm, suggesting the unbinding of one or more octamers from the DNA tether. We also observed histone octamer unbinding events at forces as low as ~2 pN. Our horizontal magnetic tweezers yielded high-resolution, low-noise data on force-mediated DNA-core histone assembly and disassembly processes.
topic single molecule micromanipulation techniques
horizontal magnetic tweezers
DNA-histone interactions
nucleosomes
url https://www.mdpi.com/1420-3049/26/16/4781
work_keys_str_mv AT robertofabian ahorizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT santoshgaire ahorizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT christophertyson ahorizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT raghabendraadhikari ahorizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT ianpegg ahorizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT abhijitsarkar ahorizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT robertofabian horizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT santoshgaire horizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT christophertyson horizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT raghabendraadhikari horizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT ianpegg horizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
AT abhijitsarkar horizontalmagnetictweezersforstudyingsinglednamoleculesanddnabindingproteins
_version_ 1721191314041602048

Similar Items