Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity.
In the context of type 1 diabetes research and the development of insulin-producing β-cell replacement strategies, whether pancreatic ductal cells retain their developmental capability to adopt an endocrine cell identity remains debated, most likely due to the diversity of models employed to induce...
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doaj-466860841aac402fac1d90c508fa37a02020-11-24T20:41:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020153610.1371/journal.pone.0201536Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity.Andhira VieiraBastien VergoniMonica CourtneyNoémie DruelleElisabet GjernesBiljana HadzicFabio AvolioTiziana NapolitanoSergi Navarro SanzAhmed MansouriPatrick CollombatIn the context of type 1 diabetes research and the development of insulin-producing β-cell replacement strategies, whether pancreatic ductal cells retain their developmental capability to adopt an endocrine cell identity remains debated, most likely due to the diversity of models employed to induce pancreatic regeneration. In this work, rather than injuring the pancreas, we developed a mouse model allowing the inducible misexpression of the proendocrine gene Neurog3 in ductal cells in vivo. These animals developed a progressive islet hypertrophy attributed to a proportional increase in all endocrine cell populations. Lineage tracing experiments indicated a continuous neo-generation of endocrine cells exhibiting a ductal ontogeny. Interestingly, the resulting supplementary β-like cells were found to be functional. Based on these findings, we suggest that ductal cells could represent a renewable source of new β-like cells and that strategies aiming at controlling the expression of Neurog3, or of its molecular targets/co-factors, may pave new avenues for the improved treatments of diabetes.http://europepmc.org/articles/PMC6084906?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andhira Vieira Bastien Vergoni Monica Courtney Noémie Druelle Elisabet Gjernes Biljana Hadzic Fabio Avolio Tiziana Napolitano Sergi Navarro Sanz Ahmed Mansouri Patrick Collombat |
spellingShingle |
Andhira Vieira Bastien Vergoni Monica Courtney Noémie Druelle Elisabet Gjernes Biljana Hadzic Fabio Avolio Tiziana Napolitano Sergi Navarro Sanz Ahmed Mansouri Patrick Collombat Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity. PLoS ONE |
author_facet |
Andhira Vieira Bastien Vergoni Monica Courtney Noémie Druelle Elisabet Gjernes Biljana Hadzic Fabio Avolio Tiziana Napolitano Sergi Navarro Sanz Ahmed Mansouri Patrick Collombat |
author_sort |
Andhira Vieira |
title |
Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity. |
title_short |
Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity. |
title_full |
Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity. |
title_fullStr |
Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity. |
title_full_unstemmed |
Neurog3 misexpression unravels mouse pancreatic ductal cell plasticity. |
title_sort |
neurog3 misexpression unravels mouse pancreatic ductal cell plasticity. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
In the context of type 1 diabetes research and the development of insulin-producing β-cell replacement strategies, whether pancreatic ductal cells retain their developmental capability to adopt an endocrine cell identity remains debated, most likely due to the diversity of models employed to induce pancreatic regeneration. In this work, rather than injuring the pancreas, we developed a mouse model allowing the inducible misexpression of the proendocrine gene Neurog3 in ductal cells in vivo. These animals developed a progressive islet hypertrophy attributed to a proportional increase in all endocrine cell populations. Lineage tracing experiments indicated a continuous neo-generation of endocrine cells exhibiting a ductal ontogeny. Interestingly, the resulting supplementary β-like cells were found to be functional. Based on these findings, we suggest that ductal cells could represent a renewable source of new β-like cells and that strategies aiming at controlling the expression of Neurog3, or of its molecular targets/co-factors, may pave new avenues for the improved treatments of diabetes. |
url |
http://europepmc.org/articles/PMC6084906?pdf=render |
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