LACK OF SPECIFICITY OF THE MOLECULAR DIAGNOSTIC METHOD FOR IDENTIFICATION OF APHANOMYCES ASTACI

LACK OF SPECIFICITY OF THE MOLECULAR DIAGNOSTIC METHOD FOR IDENTIFICATION OF APHANOMYCES ASTACI

A recent PCR-test developed for identification of Aphanomyces astaci, the organism responsible for crayfish plague, provided false positives for Aphanomyces frigidophilus, Aphanomyces repetans, and some Saprolegnia spp. Real-time PCR showed that with the designed primers, A. astaci and A. frigi...

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Bibliographic Details
Main Authors: BALLESTEROS I., MARTÍN M. P., CERENIUS L., SÖDERHÄLL K., TELLERÍA M. T., DIÉGUEZ-URIBEONDO J.
Format: Article
Language:English
Published: EDP Sciences 2007-04-01
Series:Knowledge and Management of Aquatic Ecosystems
Subjects:
Oomycetes
crayfish plague
ITS
crayfish
Conservation
invasive species
Online Access:http://dx.doi.org/10.1051/kmae:2007002
Description
Summary:A recent PCR-test developed for identification of Aphanomyces astaci, the organism responsible for crayfish plague, provided false positives for Aphanomyces frigidophilus, Aphanomyces repetans, and some Saprolegnia spp. Real-time PCR showed that with the designed primers, A. astaci and A. frigidophilus cannot be distinguished. The results of this study show that this particular crayfish plague PCR-test ought to be improved and that molecular-based techniques need to be contrasted to histological evidences and disease history.
ISSN:1961-9502

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