Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system

The maximum titre of therapeutic viral vectors can be adversely affected by the encoded transgene. Here the authors repress transgene expression in producing cells by employing the tryptophan RNA-binding attenuation protein and show that it improves titre of RNA- and DNA-based viral vectors expressi...

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Main Authors:	H. E. Maunder, J. Wright, B. R. Kolli, C. R. Vieira, T. T. Mkandawire, S. Tatoris, V. Kennedy, S. Iqball, G. Devarajan, S. Ellis, Y. Lad, N. G. Clarkson, K. A. Mitrophanous, D. C. Farley
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-03-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms14834

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spelling	doaj-461f4560315c4da1a66c68b9087e432c2021-05-11T07:17:50ZengNature Publishing GroupNature Communications2041-17232017-03-018111310.1038/ncomms14834Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) systemH. E. Maunder0J. Wright1B. R. Kolli2C. R. Vieira3T. T. Mkandawire4S. Tatoris5V. Kennedy6S. Iqball7G. Devarajan8S. Ellis9Y. Lad10N. G. Clarkson11K. A. Mitrophanous12D. C. Farley13Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.Research Department, Oxford BioMedica Ltd.The maximum titre of therapeutic viral vectors can be adversely affected by the encoded transgene. Here the authors repress transgene expression in producing cells by employing the tryptophan RNA-binding attenuation protein and show that it improves titre of RNA- and DNA-based viral vectors expressing toxic transgenes.https://doi.org/10.1038/ncomms14834
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	H. E. Maunder J. Wright B. R. Kolli C. R. Vieira T. T. Mkandawire S. Tatoris V. Kennedy S. Iqball G. Devarajan S. Ellis Y. Lad N. G. Clarkson K. A. Mitrophanous D. C. Farley
spellingShingle	H. E. Maunder J. Wright B. R. Kolli C. R. Vieira T. T. Mkandawire S. Tatoris V. Kennedy S. Iqball G. Devarajan S. Ellis Y. Lad N. G. Clarkson K. A. Mitrophanous D. C. Farley Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system Nature Communications
author_facet	H. E. Maunder J. Wright B. R. Kolli C. R. Vieira T. T. Mkandawire S. Tatoris V. Kennedy S. Iqball G. Devarajan S. Ellis Y. Lad N. G. Clarkson K. A. Mitrophanous D. C. Farley
author_sort	H. E. Maunder
title	Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system
title_short	Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system
title_full	Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system
title_fullStr	Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system
title_full_unstemmed	Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system
title_sort	enhancing titres of therapeutic viral vectors using the transgene repression in vector production (trip) system
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-03-01
description	The maximum titre of therapeutic viral vectors can be adversely affected by the encoded transgene. Here the authors repress transgene expression in producing cells by employing the tryptophan RNA-binding attenuation protein and show that it improves titre of RNA- and DNA-based viral vectors expressing toxic transgenes.
url	https://doi.org/10.1038/ncomms14834
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Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system

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