Actin Distribution in Lamina Neuralis During Cranial Neurulation of Wistar Rats Embryo (Rattus rattus)

• Main Authors: Indriayuni Prahastuti, Issoegianti S.M. R
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/7797

such as craniorachisis and exencephaly. One of the processes is changing in lamina neuralis cells shape, which is<br />caused by actin microfilament rearrangement within lamina neuralis cells. To examine the distribution of actin<br />microfilament during cranial neurulation Wistar rats embryo were used. Embryos were obtain at following days of<br />development; 8 days 18 hours, 9 days, 9 days 6 hours, 9 days 12 hours, 9 days 18 hours, and 9 days 20 hours<br />respectively. Immunohistochemistry Avidin Biotin-peroxidase Complex (ABC) method was used to examine and<br />identify the distribution of actin in lamina neuralis cells. Light microscopic observation shows positive reaction for<br />actin immunoreactivity in the apical surface of bending lamina neuralis cells. In contrast, actin is not observed in nonbending<br />lamina neuralis. Actin is not detected at 8 days 18 hours embryos. At 9 days embryos, positive reaction is<br />observed over the entire apical surface of lamina neuralis.<br />Key words: Cranial neurulation, Actin, lamina neuralis, Rats embryo.