A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis

A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis

A miniaturized optoelectronic sensor is demonstrated that measures total protein concentration in serum and urine with sensitivity and accuracy comparable to gold-standard methods. The sensor is comprised of a vertical cavity surface emitting laser (VCSEL), photodetector and other custom optical com...

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Main Authors: Ophir Vermesh, Fariah Mahzabeen, Jelena Levi, Marilyn Tan, Israt S. Alam, Carmel T. Chan, Sanjiv S. Gambhir, James S. Harris
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:MethodsX
Subjects:
VCSEL Biosensor Design and PIFE-Based Total Protein Analysis
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016121002077
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spelling doaj-461560c9a35046d290ac9f225737d45e2021-06-21T04:24:22ZengElsevierMethodsX2215-01612021-01-018101414A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysisOphir Vermesh0Fariah Mahzabeen1Jelena Levi2Marilyn Tan3Israt S. Alam4Carmel T. Chan5Sanjiv S. Gambhir6James S. Harris7Department of Radiology, Stanford University, Stanford, CA, 94305, USA; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USA; Corresponding authors.Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USADepartment of Radiology, Stanford University, Stanford, CA, 94305, USA; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USADepartment of Medicine, Stanford University, Stanford, CA 94305, USADepartment of Radiology, Stanford University, Stanford, CA, 94305, USA; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USADepartment of Radiology, Stanford University, Stanford, CA, 94305, USA; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USADepartment of Radiology, Stanford University, Stanford, CA, 94305, USA; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Stanford Bio-X, Stanford University, Stanford, CA 94305, USADepartment of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA; Corresponding authors.A miniaturized optoelectronic sensor is demonstrated that measures total protein concentration in serum and urine with sensitivity and accuracy comparable to gold-standard methods. The sensor is comprised of a vertical cavity surface emitting laser (VCSEL), photodetector and other custom optical components and electronics that can be hybrid packaged into a portable, handheld form factor. In conjunction, a custom fluorescence assay has been developed based on the protein-induced fluorescence enhancement (PIFE) phenomenon, enabling real-time sensor response to changes in protein concentration. Methods are described for the following: • Standard curves: Used to determine the sensitivity, dynamic range, and linearity of the VCSEL biosensor/PIFE assay system in buffer as well as in human blood and urine samples. • Comparison of VCSEL biosensor performance with a benchtop fluorimetric microplate reader. • Accuracy of the VCSEL biosensor/PIFE assay system: Evaluated by comparing sensor measurements with gold-standard clinical laboratory measurements of total protein in serum and urine samples from patients with diabetes.http://www.sciencedirect.com/science/article/pii/S2215016121002077VCSEL Biosensor Design and PIFE-Based Total Protein Analysis
collection DOAJ
language English
format Article
sources DOAJ
author Ophir Vermesh
Fariah Mahzabeen
Jelena Levi
Marilyn Tan
Israt S. Alam
Carmel T. Chan
Sanjiv S. Gambhir
James S. Harris
spellingShingle Ophir Vermesh
Fariah Mahzabeen
Jelena Levi
Marilyn Tan
Israt S. Alam
Carmel T. Chan
Sanjiv S. Gambhir
James S. Harris
A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis
MethodsX
VCSEL Biosensor Design and PIFE-Based Total Protein Analysis
author_facet Ophir Vermesh
Fariah Mahzabeen
Jelena Levi
Marilyn Tan
Israt S. Alam
Carmel T. Chan
Sanjiv S. Gambhir
James S. Harris
author_sort Ophir Vermesh
title A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis
title_short A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis
title_full A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis
title_fullStr A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis
title_full_unstemmed A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis
title_sort miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis
publisher Elsevier
series MethodsX
issn 2215-0161
publishDate 2021-01-01
description A miniaturized optoelectronic sensor is demonstrated that measures total protein concentration in serum and urine with sensitivity and accuracy comparable to gold-standard methods. The sensor is comprised of a vertical cavity surface emitting laser (VCSEL), photodetector and other custom optical components and electronics that can be hybrid packaged into a portable, handheld form factor. In conjunction, a custom fluorescence assay has been developed based on the protein-induced fluorescence enhancement (PIFE) phenomenon, enabling real-time sensor response to changes in protein concentration. Methods are described for the following: • Standard curves: Used to determine the sensitivity, dynamic range, and linearity of the VCSEL biosensor/PIFE assay system in buffer as well as in human blood and urine samples. • Comparison of VCSEL biosensor performance with a benchtop fluorimetric microplate reader. • Accuracy of the VCSEL biosensor/PIFE assay system: Evaluated by comparing sensor measurements with gold-standard clinical laboratory measurements of total protein in serum and urine samples from patients with diabetes.
topic VCSEL Biosensor Design and PIFE-Based Total Protein Analysis
url http://www.sciencedirect.com/science/article/pii/S2215016121002077
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