The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination

• Main Authors: Buntić Aneta V., Stajković-Srbinović Olivera S., Knežević Magdalena M., Rasulić Nataša I., Kuzmanović Đorđe Ž., Dimitrijević-Branković Suzana I., Delić Dušica I.
• Format: Article
• Language: English
• Published: Serbian Soil Science Society, Belgrade 2019-01-01
• Series: Zemljište i biljka
• Subjects: bacillus sp.; seed germination; indole-3-acetic acid (iaa); siderophores; triticum aestivum; hordeum jubatum
• Online Access: https://scindeks-clanci.ceon.rs/data/pdf/0514-6658/2019/0514-66581902001B.pdf
• ISSN: 0514-6658; 2560-4279

Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion of plant growth. The most comprehensively studied bacteria with these effects belong to the Bacillus genus. In this study, seven Bacillus isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics of these isolates, such as production of indole-3aceticacid, siderophores and hydrogen cyanide were tested. The induction of wheat (Triticum aestivum) and barley (Hordeum jubatum) seed germination was evaluated in vitro. Isolate BMG2 produced the highest indole-3-aceticacid of 24.89 µg/mL. The length of roots of barley increased up to 60%, while the length of shoots of barley increased 2.23 times afterapplying isolatesPAZE-6 and BMG1, respectively. In addition, isolates BMG1, BMG2 and PAZE-6 improved germination of both types of seeds and showed ability to produce useful substances such as siderophores and indole-3-acetic acid. Further, these isolates could be used in the production of liquid crop additives that can improve the total yield of cultivated plants, especially barley.