Competition NMR for Detection of Hit/Lead Inhibitors of Protein–Protein Interactions

Competition NMR for Detection of Hit/Lead Inhibitors of Protein–Protein Interactions

Screening for small-molecule fragments that can lead to potent inhibitors of protein–protein interactions (PPIs) is often a laborious step as the fragments cannot dissociate the targeted PPI due to their low μM–mM affinities. Here, we describe an NMR competition assay called w-AIDA-NMR (weak-antagon...

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Bibliographic Details
Main Authors: Bogdan Musielak, Weronika Janczyk, Ismael Rodriguez, Jacek Plewka, Dominik Sala, Katarzyna Magiera-Mularz, Tad Holak
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Molecules
Subjects:
NMR
Mdm2/p53
PD-1/PD-L1
protein–protein interaction
small molecule
Online Access:https://www.mdpi.com/1420-3049/25/13/3017

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