piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice
SUMMARY The stable genomic integration and expression of a large transgene is a major hurdle in gene therapy. We show that the modified piggyBac (PB) transposon system can be used to introduce a 207 kb genomic DNA fragment containing the RORγ/γt locus into human cells and mice. PB-mediated transgene...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2013-05-01
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| Series: | Disease Models & Mechanisms |
| Online Access: | http://dmm.biologists.org/content/6/3/828 |