Anti-nociceptive and anti-inflammatory activities of Tetracarpidium conophorum seed lectin

• Main Authors: Boniface Oladiran Oladokun, Omotayo Nusirat Omisore, Olukemi Adetutu Osukoya, Adenike Kuku
• Format: Article
• Language: English
• Published: Elsevier 2019-05-01
• Series: Scientific African
• Online Access: http://www.sciencedirect.com/science/article/pii/S2468227618303582

The anti-nociceptive and anti-inflammatory activity of the lectin purified from Tetracarpidium conophorum seeds were investigated.The lectin was purified from the phosphate buffered saline crude extract of the seeds using gel filtration and affinity chromatography techniques. Hemagglutination assay and hapten-sugar inhibition test were carried out to ascertain the blood group and sugar specificities of the lectin respectively. Anti-nociceptive and anti-inflammatory activities of the lectin were evaluated (in-vivo) with standard models including formalin licking test, carrageenan-induced paw edema test and peritonitis model.A yield, purification fold and subunit molecular weight of 27%, 17 and 34 kDa respectively were obtained for the purified lectin from T. conophorum seed (TcSL). The lactose/galactose specific TcSL showed significant inhibition (p < 0.05) of nociception as measured by paw licking time upon pain induction by formalin and inflammation as measured by paw swollenness induced by sub-plantar injection of carrageenan. The lectin also significantly reduced carrageenan-induced leucocyte migration to the peritoneum in a dose-dependent manner.The study concluded that TcSL showed anti-nociceptive and anti-inflammatory activities and thus poised to be a good bio-active peptide for the design of novel anti-inflammatory therapy. Keywords: Nociception, Inflammation, Lectin, seed, Tetracarpidium conophorum