Fluorescent Method for Observing Intravascular Bonghan Duct

Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining...

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Bibliographic Details
Main Authors: Byung-Cheon Lee, Ku Youn Baik, Hyeon-Min Johng, Baekkyoung Sung, Kyung Soon Soh, Dae-In Kang, Kwang-Sup Soh
Format: Article
Language:English
Published: Korean Pharmacopuncture Institute 2005-12-01
Series:Journal of Pharmacopuncture
Subjects:
Online Access:http://dx.doi.org/10.3831/KPI.2005.8.3.005
Description
Summary:Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.
ISSN:2093-6966
2234-6856