Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats

Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats

A rat model of secondary osteoporosis was constructed using retinoic acid as an inducer, and the genes, proteins, and bone mass of the rats were analyzed. qPCR detection of the Wnt/β-catenin and OPG/RANK/RANKL signaling pathway-related gene expression levels showed that Lactobacillus plantarum HFY15...

Full description

Bibliographic Details
Main Authors: Xinhong Liu, Jiazhuang Zheng, Fang Li, Ruokun Yi, Jianfei Mu, Fang Tan, Xin Zhao
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2020/2054389
id doaj-45bce7a4d69b41e1821cd79728ce0f03
record_format Article
spelling doaj-45bce7a4d69b41e1821cd79728ce0f032020-11-25T03:40:32ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882020-01-01202010.1155/2020/20543892054389Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar RatsXinhong Liu0Jiazhuang Zheng1Fang Li2Ruokun Yi3Jianfei Mu4Fang Tan5Xin Zhao6Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaDepartment of Spine Surgery, Suining Center Hospital, Chongqing Medical University, Suining, Sichuan 629000, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaDepartment of Public Health, Our Lady of Fatima University, Valenzuela 838, PhilippinesChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaA rat model of secondary osteoporosis was constructed using retinoic acid as an inducer, and the genes, proteins, and bone mass of the rats were analyzed. qPCR detection of the Wnt/β-catenin and OPG/RANK/RANKL signaling pathway-related gene expression levels showed that Lactobacillus plantarum HFY15 played a positive role in regulating both pathways. HFY15 significantly increased β-catenin, Lrp5, Lrp6, Wnt10b, OPG, RANKL, and Runx2 expression and downregulated DKK1, RANK, CTSK, TRACP, and ALP expression. Enzyme-linked immunosorbent assays further confirmed the qPCR results. Tartrate-resistant acid phosphatase staining showed that HFY15 slowed retinoic acid-induced osteoclast formation. Microcomputed tomography showed that HFY15 reduced trabecular separation and increased the percent bone volume, trabecular numbers, trabecular thickness, and bone mineral density in the rats in vivo. These findings indicate that HFY15 may help prevent retinoic acid-induced secondary osteoporosis in vivo.http://dx.doi.org/10.1155/2020/2054389
collection DOAJ
language English
format Article
sources DOAJ
author Xinhong Liu
Jiazhuang Zheng
Fang Li
Ruokun Yi
Jianfei Mu
Fang Tan
Xin Zhao
spellingShingle Xinhong Liu
Jiazhuang Zheng
Fang Li
Ruokun Yi
Jianfei Mu
Fang Tan
Xin Zhao
Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats
Evidence-Based Complementary and Alternative Medicine
author_facet Xinhong Liu
Jiazhuang Zheng
Fang Li
Ruokun Yi
Jianfei Mu
Fang Tan
Xin Zhao
author_sort Xinhong Liu
title Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats
title_short Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats
title_full Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats
title_fullStr Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats
title_full_unstemmed Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats
title_sort lactobacillus plantarum hfy15 helps prevent retinoic acid-induced secondary osteoporosis in wistar rats
publisher Hindawi Limited
series Evidence-Based Complementary and Alternative Medicine
issn 1741-427X
1741-4288
publishDate 2020-01-01
description A rat model of secondary osteoporosis was constructed using retinoic acid as an inducer, and the genes, proteins, and bone mass of the rats were analyzed. qPCR detection of the Wnt/β-catenin and OPG/RANK/RANKL signaling pathway-related gene expression levels showed that Lactobacillus plantarum HFY15 played a positive role in regulating both pathways. HFY15 significantly increased β-catenin, Lrp5, Lrp6, Wnt10b, OPG, RANKL, and Runx2 expression and downregulated DKK1, RANK, CTSK, TRACP, and ALP expression. Enzyme-linked immunosorbent assays further confirmed the qPCR results. Tartrate-resistant acid phosphatase staining showed that HFY15 slowed retinoic acid-induced osteoclast formation. Microcomputed tomography showed that HFY15 reduced trabecular separation and increased the percent bone volume, trabecular numbers, trabecular thickness, and bone mineral density in the rats in vivo. These findings indicate that HFY15 may help prevent retinoic acid-induced secondary osteoporosis in vivo.
url http://dx.doi.org/10.1155/2020/2054389
work_keys_str_mv AT xinhongliu lactobacillusplantarumhfy15helpspreventretinoicacidinducedsecondaryosteoporosisinwistarrats
AT jiazhuangzheng lactobacillusplantarumhfy15helpspreventretinoicacidinducedsecondaryosteoporosisinwistarrats
AT fangli lactobacillusplantarumhfy15helpspreventretinoicacidinducedsecondaryosteoporosisinwistarrats
AT ruokunyi lactobacillusplantarumhfy15helpspreventretinoicacidinducedsecondaryosteoporosisinwistarrats
AT jianfeimu lactobacillusplantarumhfy15helpspreventretinoicacidinducedsecondaryosteoporosisinwistarrats
AT fangtan lactobacillusplantarumhfy15helpspreventretinoicacidinducedsecondaryosteoporosisinwistarrats
AT xinzhao lactobacillusplantarumhfy15helpspreventretinoicacidinducedsecondaryosteoporosisinwistarrats
_version_ 1715149394993479680

Similar Items