Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS

Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS

Microcystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatogr...

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Main Authors: Gaodao Liang, Ping Xie, Jun Chen, Ting Yu
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2011.17
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spelling doaj-45b1ae62b4e846f3b1fa716041dfb6ad2020-11-24T22:09:24ZengHindawi LimitedThe Scientific World Journal1537-744X2011-01-0111202610.1100/tsw.2011.17Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MSGaodao Liang0Ping Xie1Jun Chen2Ting Yu3Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDonghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDonghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDonghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaMicrocystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatography combined with mass spectrometry (LC-MS). There was a remarkable difference in such relationships between MC-RR and -LR. The log DOW of MC-LR decreased from 1.63 at pH 1.0 to -1.26 at pH 6.5, and stabilized between -1.04 and -1.56 at a pH of 6.5~12.0; log DOW of MC-RR varied between -1.24 and -0.67 at a pH of 1.00~4.00, and stabilized between -1.20 and -1.54 at a pH of 4.00~12.00. The difference of hydrophobicity in acidic condition between MC-RR and -LR is important, not only for the analytical method of both toxins, but perhaps also for understanding the difference of toxicity to animals between the two toxins.http://dx.doi.org/10.1100/tsw.2011.17
collection DOAJ
language English
format Article
sources DOAJ
author Gaodao Liang
Ping Xie
Jun Chen
Ting Yu
spellingShingle Gaodao Liang
Ping Xie
Jun Chen
Ting Yu
Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS
The Scientific World Journal
author_facet Gaodao Liang
Ping Xie
Jun Chen
Ting Yu
author_sort Gaodao Liang
title Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS
title_short Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS
title_full Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS
title_fullStr Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS
title_full_unstemmed Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS
title_sort comparative studies on the ph dependence of dow of microcystin-rr and -lr using lc-ms
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2011-01-01
description Microcystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatography combined with mass spectrometry (LC-MS). There was a remarkable difference in such relationships between MC-RR and -LR. The log DOW of MC-LR decreased from 1.63 at pH 1.0 to -1.26 at pH 6.5, and stabilized between -1.04 and -1.56 at a pH of 6.5~12.0; log DOW of MC-RR varied between -1.24 and -0.67 at a pH of 1.00~4.00, and stabilized between -1.20 and -1.54 at a pH of 4.00~12.00. The difference of hydrophobicity in acidic condition between MC-RR and -LR is important, not only for the analytical method of both toxins, but perhaps also for understanding the difference of toxicity to animals between the two toxins.
url http://dx.doi.org/10.1100/tsw.2011.17
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AT pingxie comparativestudiesonthephdependenceofdowofmicrocystinrrandlrusinglcms
AT junchen comparativestudiesonthephdependenceofdowofmicrocystinrrandlrusinglcms
AT tingyu comparativestudiesonthephdependenceofdowofmicrocystinrrandlrusinglcms
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