Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic Guidance

Khaled Z Alawneh,1 Liqaa A Raffee,2 Musa Ahmed Mohammed Alshehabat,3 Hazem Haddad,4 Saied A Jaradat5 1Department of Diagnostic Radiology and Nuclear Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan; 2Department of Accident and Emergency Medicine, Faculty of M...

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Main Authors: Alawneh KZ, Raffee La, Alshehabat MAM, Haddad H, Jaradat SA
Format: Article
Language:English
Published: Dove Medical Press 2021-09-01
Series:Vascular Health and Risk Management
Subjects:
Online Access:https://www.dovepress.com/characterizing-and-profiling-micrornas-in-dogs-undergoing-induced-isch-peer-reviewed-fulltext-article-VHRM
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spelling doaj-45ab318ca3fd4bf5bd28908b4f7811162021-09-09T19:31:53ZengDove Medical PressVascular Health and Risk Management1178-20482021-09-01Volume 1754355068657Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic GuidanceAlawneh KZRaffee LaAlshehabat MAMHaddad HJaradat SAKhaled Z Alawneh,1 Liqaa A Raffee,2 Musa Ahmed Mohammed Alshehabat,3 Hazem Haddad,4 Saied A Jaradat5 1Department of Diagnostic Radiology and Nuclear Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan; 2Department of Accident and Emergency Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan; 3Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan; 4Haya Biotechnology Center, Jordan University of Science and Technology, Irbid, 22110, Jordan; 5Princess Haya Biotechnology Center, Jordan University of Science and Technology, Irbid, 22110, JordanCorrespondence: Liqaa A RaffeeDepartment of Accident and Emergency Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, JordanEmail laraffee5@just.edu.joPurpose: Ischemic stroke of the brain is still considered one of the most common causes of disability and death in developed and developing countries in human beings despite advances in medicine and technology. This study was conducted to characterize and profile tens of induced biomarkers (microRNAs) after experimentally inducing regional ischemic stroke of the brain by occluding the middle cerebral artery under fluoroscopic guidance using an autologous blood clot.Patient and Methods: A total of six healthy dogs were recruited for this study. The microRNAs were profiled in the blood and urine before and after occluding the middle cerebral artery using genetic techniques.Results: The very highly expressed genes were comprised within cluster A, followed by cluster D in both 24 and 48-hour brain samples. Clusters B and C revealed down-regulated genes, while miRNAs remained up-regulated in the 24-hour samples merely in cluster F. Upregulated genes at 48 hours of reperfusion were included in cluster E. On the other hand, changes were observed after a day on the cluster G genes. Exclusive upregulation was notified after 2 days due to the changes in mIR-138. The normalized gene expression in the test sample is witnessed through Fold-Change, which divides the control sample’s normalized gene expression. Moreover, fold-change has emerged as a significant approach for representing fold-regulation.Conclusion: The microRNAs expression in blood and urine may have a potential role in the diagnosis, prognosis, and assessment of therapy associated with cerebral artery occlusion under fluoroscopic guidance.Keywords: dogs, fluoroscopic induced ischemic brain, microRNAs, middle cerebral artery strokehttps://www.dovepress.com/characterizing-and-profiling-micrornas-in-dogs-undergoing-induced-isch-peer-reviewed-fulltext-article-VHRMdogsfluoroscopic induced ischemic brainmicrornasmiddle cerebral artery stroke
collection DOAJ
language English
format Article
sources DOAJ
author Alawneh KZ
Raffee La
Alshehabat MAM
Haddad H
Jaradat SA
spellingShingle Alawneh KZ
Raffee La
Alshehabat MAM
Haddad H
Jaradat SA
Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic Guidance
Vascular Health and Risk Management
dogs
fluoroscopic induced ischemic brain
micrornas
middle cerebral artery stroke
author_facet Alawneh KZ
Raffee La
Alshehabat MAM
Haddad H
Jaradat SA
author_sort Alawneh KZ
title Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic Guidance
title_short Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic Guidance
title_full Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic Guidance
title_fullStr Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic Guidance
title_full_unstemmed Characterizing and Profiling microRNAs in Dogs Undergoing Induced Ischemic Brain Stroke After Middle Cerebral Artery Occlusion Under Fluoroscopic Guidance
title_sort characterizing and profiling micrornas in dogs undergoing induced ischemic brain stroke after middle cerebral artery occlusion under fluoroscopic guidance
publisher Dove Medical Press
series Vascular Health and Risk Management
issn 1178-2048
publishDate 2021-09-01
description Khaled Z Alawneh,1 Liqaa A Raffee,2 Musa Ahmed Mohammed Alshehabat,3 Hazem Haddad,4 Saied A Jaradat5 1Department of Diagnostic Radiology and Nuclear Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan; 2Department of Accident and Emergency Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan; 3Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan; 4Haya Biotechnology Center, Jordan University of Science and Technology, Irbid, 22110, Jordan; 5Princess Haya Biotechnology Center, Jordan University of Science and Technology, Irbid, 22110, JordanCorrespondence: Liqaa A RaffeeDepartment of Accident and Emergency Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, JordanEmail laraffee5@just.edu.joPurpose: Ischemic stroke of the brain is still considered one of the most common causes of disability and death in developed and developing countries in human beings despite advances in medicine and technology. This study was conducted to characterize and profile tens of induced biomarkers (microRNAs) after experimentally inducing regional ischemic stroke of the brain by occluding the middle cerebral artery under fluoroscopic guidance using an autologous blood clot.Patient and Methods: A total of six healthy dogs were recruited for this study. The microRNAs were profiled in the blood and urine before and after occluding the middle cerebral artery using genetic techniques.Results: The very highly expressed genes were comprised within cluster A, followed by cluster D in both 24 and 48-hour brain samples. Clusters B and C revealed down-regulated genes, while miRNAs remained up-regulated in the 24-hour samples merely in cluster F. Upregulated genes at 48 hours of reperfusion were included in cluster E. On the other hand, changes were observed after a day on the cluster G genes. Exclusive upregulation was notified after 2 days due to the changes in mIR-138. The normalized gene expression in the test sample is witnessed through Fold-Change, which divides the control sample’s normalized gene expression. Moreover, fold-change has emerged as a significant approach for representing fold-regulation.Conclusion: The microRNAs expression in blood and urine may have a potential role in the diagnosis, prognosis, and assessment of therapy associated with cerebral artery occlusion under fluoroscopic guidance.Keywords: dogs, fluoroscopic induced ischemic brain, microRNAs, middle cerebral artery stroke
topic dogs
fluoroscopic induced ischemic brain
micrornas
middle cerebral artery stroke
url https://www.dovepress.com/characterizing-and-profiling-micrornas-in-dogs-undergoing-induced-isch-peer-reviewed-fulltext-article-VHRM
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