EVALUATION OF TWO in vitro MATURATION MEDIUM FOR EMBRYO PRODUCTION IN SHEEP

• Main Authors: J. M. Robledo Verduzco, José Herrera Camacho, M. Cajero Juaréz, M. C. Navarro Maldonado, A. García Valladares
• Format: Article
• Language: English
• Published: Universidad Autónoma de Yucatán 2008-12-01
• Series: Tropical and Subtropical Agroecosystems
• Subjects: Maturation; fertilization; oocytes; embryos; ovine
• Online Access: http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/49

The aim of this work was to evaluate the effect of HECM-9 and TCM-199 as maturation media on maturation (MR), in vitro fertilization (IVF) and embryo development (ED) rate of oocytes from hair sheep collected from slaughter house ovaries. Cumulus-oocyte-complexes (COC) were obtained by manual aspiration, from 2-6mm diameter follicles. Groups of 10-20 COC, quality 1 and 2 were placed into 450μL of HECM-9 or TCM-199 and incubated 24h at 38.5 ºC and 5% CO2. For IVF, COC were transferred to 450μL of SOFm+Oaa, 0.5 x 106 motile spermatozoa were added and then incubated at 38.5 ºC and 5% CO2. Alleged zygotes were transferred to 450μL of SOFm+Oaa+glucose. Embryo development and morphology were evaluated at 2, 4, 5 and 6 days of culture, not developed zygotes were removed on day 2 and the final rate of embryo production was determined on day 8 of culture. Oocyte MR showed no significant differences (P>0.05) between treatments (73.3 vs. 71.4 % HECM-9 and TCM-199, respectively). Fertilization rate was different (P