ANALISA BEBERAPA PARAMETER MOTILITAS SPERMATOZOA PADA BERBAGAI BANGSA SAPI MENGGUNAKAN COMPUTER ASSISTED SEMEN ANALYSIS (CASA)

• Main Authors: Sarastina Sarastina, T. Susilawati, G. Ciptadi
• Format: Article
• Language: Indonesian
• Published: University of Brawijaya 2012-05-01
• Series: Jurnal Ternak Tropika
• Online Access: http://ternaktropika.ub.ac.id/index.php/tropika/article/view/146

ABSTRAK Tujuan Penelitian ini adalah untuk mempelajari 18 parameter motilitas spermatozoa menggunakan metode CASA serta motilitas progresif secara visual dibandingkan ddengan metoda CASA. Materi adalah semen segar dari 8 bangsa sapi yaitu Limousin, Bali Madura, Simental, Brahman, dan Ongole. Sata dianalisa dengan analisis varian, sementara data motilitas progresif diuji t-test. Hasil menunjukkan bahwa motilitas progresif menggunakan CASA tidak berbeda nyata (P>0.05) diantara 6 bangsa sapi. Sementara itu parameter motilitas hiperaktif menggunakan CASA tidak berbeda nyata (P>0.05) kecuali on linier, non limear, juga parameter: DCL, DAP, DSL, VCL, VAP, VSL, LIN< STR< WOB, ALH, AOH adalah berbeda nyata (P<0.05) diantara bangsa sapi. Uji t pada motilitas progresif menunjukkan tidak ada beda nyata (P>0.05) antara CASA dengan visula. Kata kunci: Motilitas, CASA, spermatozoa, sapi. ABSTRACT The aims of this research were to determine 18 parameters spermatozoa motility using CASA method and to determine spermatozoa progressive motility using visual assessment and CASA. The material of this research were bull fresh semen from 6 breeds, there were Limmousin, Bali, Madura, Simmental, Brahman and Ongole which owned by National Artificial Insemination Center Singosari. Data were collected from CASA method of 18 parameters sperm motility and visual assessment method of progressive sperm motility. Data were analyzed by variance and factor analysis, and progressive motility using visual and CASA were analyzed by paired TTest. The result showed that motility and progressive motility using CASA was no significant different (P>0.05) for six breeds. There were no significant different (P>0.05) on hyperactive parameter using CASA, but on linear, non linear, curve linear and cell detail parameter : DCL, DAP, DSL, VCL, VAP, VSL, LIN, STR, WOB, ALH, AOH were significant different (P<0.05) among breeds. Analysis using paired T-test of progressive motility assessment was no signifgicant different between CASA and visual microscopic (P>0.05). Key words : Motility, spermatozoa, CASA, breeds