Coupling Microplate-Based Antibacterial Assay with Liquid Chromatography for High-Resolution Growth Inhibition Profiling of Crude Extracts: Validation and Proof-of-Concept Study with <i>Staphylococcus aureus</i>

Coupling Microplate-Based Antibacterial Assay with Liquid Chromatography for High-Resolution Growth Inhibition Profiling of Crude Extracts: Validation and Proof-of-Concept Study with <i>Staphylococcus aureus</i>

With the identification of novel antibiotics from nature being pivotal in the fight against human pathogenic bacteria, there is an urgent need for effective methodologies for expedited screening of crude extracts. Here we report the development and validation of a simple and dye-free antimicrobial a...

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Bibliographic Details
Main Authors: Hamidreza Ardalani, Syariful Anam, Kresten J.K. Kromphardt, Dan Staerk, Kenneth T. Kongstad
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Molecules
Subjects:
dye-free microplate assay
antibacterial
high-resolution antibacterial profiling
natural products
<i>Staphylococcus aureus</i>
Online Access:https://www.mdpi.com/1420-3049/26/6/1550

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