Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter

Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter

Cellwall invertase (CWIN) hydrolyzes sucrose into glucose and fructose irreversibly, playing key roles in carbohydrate partitioning and plant defence. MeCWINV1 is one of the CWINs in cassava, which contains several light-responsive elements and stress-responsive elements in promoter region. To analy...

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Main Authors: Liu Jiao, Guo Yuqiang, Duan Ruijun, Wang Shuo, Fu Shaoping, Kong Guanghong, Li Ruimei, Yao Yuan, Guo Jianchun
Format: Article
Language:English
Published: EDP Sciences 2017-01-01
Series:BIO Web of Conferences
Online Access:http://dx.doi.org/10.1051/bioconf/20170803020
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spelling doaj-4502bfeda4134eea949ae705c90f620f2021-04-02T18:52:23ZengEDP SciencesBIO Web of Conferences2117-44582017-01-0180302010.1051/bioconf/20170803020bioconf_icmsb2017_03020Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 PromoterLiu Jiao0Guo YuqiangDuan Ruijun1Wang ShuoFu Shaoping2Kong GuanghongLi Ruimei3Yao Yuan4Guo Jianchun5Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureCellwall invertase (CWIN) hydrolyzes sucrose into glucose and fructose irreversibly, playing key roles in carbohydrate partitioning and plant defence. MeCWINV1 is one of the CWINs in cassava, which contains several light-responsive elements and stress-responsive elements in promoter region. To analyze the regulatory function of MeCWINV1 in the cassava starch accumulation and stress defense response, a 865 bp MeCWINV1 promoter fragment was cloned and inserted into yeast one-hybrid bait vector to construct pCW1-AbAi vector, then was transformed to Y1HGold yeast strains to screen the binding proteins. It might provide a framework for further investigation on the regulation mechanism of MeCWINV1 gene in cassava.http://dx.doi.org/10.1051/bioconf/20170803020
collection DOAJ
language English
format Article
sources DOAJ
author Liu Jiao
Guo Yuqiang
Duan Ruijun
Wang Shuo
Fu Shaoping
Kong Guanghong
Li Ruimei
Yao Yuan
Guo Jianchun
spellingShingle Liu Jiao
Guo Yuqiang
Duan Ruijun
Wang Shuo
Fu Shaoping
Kong Guanghong
Li Ruimei
Yao Yuan
Guo Jianchun
Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter
BIO Web of Conferences
author_facet Liu Jiao
Guo Yuqiang
Duan Ruijun
Wang Shuo
Fu Shaoping
Kong Guanghong
Li Ruimei
Yao Yuan
Guo Jianchun
author_sort Liu Jiao
title Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter
title_short Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter
title_full Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter
title_fullStr Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter
title_full_unstemmed Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter
title_sort construction of yeast one-hybrid bait reporter vector for screening the binding proteins of cassava mecwinv1 promoter
publisher EDP Sciences
series BIO Web of Conferences
issn 2117-4458
publishDate 2017-01-01
description Cellwall invertase (CWIN) hydrolyzes sucrose into glucose and fructose irreversibly, playing key roles in carbohydrate partitioning and plant defence. MeCWINV1 is one of the CWINs in cassava, which contains several light-responsive elements and stress-responsive elements in promoter region. To analyze the regulatory function of MeCWINV1 in the cassava starch accumulation and stress defense response, a 865 bp MeCWINV1 promoter fragment was cloned and inserted into yeast one-hybrid bait vector to construct pCW1-AbAi vector, then was transformed to Y1HGold yeast strains to screen the binding proteins. It might provide a framework for further investigation on the regulation mechanism of MeCWINV1 gene in cassava.
url http://dx.doi.org/10.1051/bioconf/20170803020
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