Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus

Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus

Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were...

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Bibliographic Details
Main Authors: Roberto B. Flatschart, Maurício Resende
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2000-10-01
Series:Brazilian Journal of Microbiology
Subjects:
vírus da doença de Aujeszky
reação em cadeia da polimerase
hibridização dot-blot
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013&lng=en&tlng=en
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spelling doaj-44fd5ec405a84e1d9b68c0c47092d9ac2020-11-24T22:53:18ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052000-10-0131430831110.1590/S1517-83822000000400013S1517-83822000000400013Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virusRoberto B. Flatschart0Maurício Resende1Universidade Federal de Minas GeraisUniversidade Federal de Minas GeraisAcute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013&lng=en&tlng=envírus da doença de Aujeszkyreação em cadeia da polimerasehibridização dot-blot
collection DOAJ
language English
format Article
sources DOAJ
author Roberto B. Flatschart
Maurício Resende
spellingShingle Roberto B. Flatschart
Maurício Resende
Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
Brazilian Journal of Microbiology
vírus da doença de Aujeszky
reação em cadeia da polimerase
hibridização dot-blot
author_facet Roberto B. Flatschart
Maurício Resende
author_sort Roberto B. Flatschart
title Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_short Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_full Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_fullStr Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_full_unstemmed Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_sort acute and latent infection in mice with a virulent strain of aujeszky’s disease virus
publisher Sociedade Brasileira de Microbiologia
series Brazilian Journal of Microbiology
issn 1678-4405
publishDate 2000-10-01
description Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
topic vírus da doença de Aujeszky
reação em cadeia da polimerase
hibridização dot-blot
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013&lng=en&tlng=en
work_keys_str_mv AT robertobflatschart acuteandlatentinfectioninmicewithavirulentstrainofaujeszkysdiseasevirus
AT mauricioresende acuteandlatentinfectioninmicewithavirulentstrainofaujeszkysdiseasevirus
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