A new extraction method of bioflavanoids from poisonous plant (Gratiola Officinalis L.)

• Main Authors: Natalya V. Polukonova, Mariya N. Kurchatova, Nikita A. Navolokin, Alla B. Bucharskaya, Natalya A. Durnova, Galina N. Maslyakova
• Format: Article
• Language: English
• Published: Limited liability company «Science and Innovations» (Saratov) 2014-09-01
• Series: Russian Open Medical Journal
• Subjects: Gratiola officinalis L; hyssop; bioflavonoids; chemical composition; extraction method; quercetin
• Online Access: http://www.romj.org/node/109

The way of vegetable raw materials extraction which allows to receive nontoxical composition of biological active agents from poisonous plants such as Gratiola officinalis L. was described. The alkaloids exit changes with the increase of ethyl alcohol percentage (from 15% to 96%). The extract was obtained using 96% ethanol and did not give positive high quality reaction to the content of alkaloids. The chemical composition with new nontoxical biological active composition of Gratiola officinalis L. extract was investigated. The extract contains a previously unknown plant – bioflavonoid quercetin. The average value of quercetin in this extract using the calibration curve of the standard sample quercetin (98%) Sigma is 0.66%. In the dry rest of extractive substances (Gratiola officinalis L.) the quantity of quercetin was 350 mkg (obtained from 10 g of a dry grass) as was established by the method of a liquid chromatography.