Absolute measurement of gene transcripts with Selfie-digital PCR

Abstract Absolute measurement of the number of RNA transcripts per gene is necessary to compare gene transcription among different tissues or experimental conditions and to assess transcription of genes that have a variable copy number per cell such as mitochondrial DNA. Here, we present a method ca...

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Bibliographic Details
Main Authors: Petar Podlesniy, Ramon Trullas
Format: Article
Language:English
Published: Nature Publishing Group 2017-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-08270-w