The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion

The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion

Our previous reports showed that the cyclic-AMP-response element-binding protein (CREB) served as a proto-oncogene in the process of tumorigenesis and mediated the growth and metastatic activity of renal cancer cells. Our study, therefore, explored the role of CREB in sorafenib- -inhibited cell prol...

Full description

Bibliographic Details
Main Authors: Huang Shuaishuai, Cui Pinger, Lin Shuangxia, Yao Xuping, Wang Xue, Ren Yu, Weng Guobin
Format: Article
Language:English
Published: Sciendo 2018-12-01
Series:Acta Pharmaceutica
Subjects:
creb
renal cancer
sorafenib
migration
invasion
Online Access:https://doi.org/10.2478/acph-2018-0033
id doaj-44ae6b31b008463ab543921ff6b5e07f
record_format Article
spelling doaj-44ae6b31b008463ab543921ff6b5e07f2021-09-06T19:40:58ZengSciendoActa Pharmaceutica1846-95582018-12-0168449750610.2478/acph-2018-0033acph-2018-0033The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and InvasionHuang Shuaishuai0Cui Pinger1Lin Shuangxia2Yao Xuping3Wang Xue4Ren Yu5Weng Guobin6Laboratory of Renal Carcinoma, Ningbo Yinzhou No. 2 Hospital, Ningbo 315100,Zhejiang, P.R., ChinaLaboratory of Renal Carcinoma, Ningbo Yinzhou No. 2 Hospital, Ningbo 315100,Zhejiang, P.R., ChinaLaboratory of Renal Carcinoma, Ningbo Yinzhou No. 2 Hospital, Ningbo 315100,Zhejiang, P.R., ChinaLaboratory of Renal Carcinoma, Ningbo Yinzhou No. 2 Hospital, Ningbo 315100,Zhejiang, P.R., ChinaLaboratory of Renal Carcinoma, Ningbo Yinzhou No. 2 Hospital, Ningbo 315100,Zhejiang, P.R., ChinaLaboratory of Renal Carcinoma, Ningbo Yinzhou No. 2 Hospital, Ningbo 315100,Zhejiang, P.R., ChinaLaboratory of Renal Carcinoma, Ningbo Yinzhou No. 2 Hospital, Ningbo 315100,Zhejiang, P.R., ChinaOur previous reports showed that the cyclic-AMP-response element-binding protein (CREB) served as a proto-oncogene in the process of tumorigenesis and mediated the growth and metastatic activity of renal cancer cells. Our study, therefore, explored the role of CREB in sorafenib- -inhibited cell proliferation, migration and invasion. Renal cancer cells were cultured in medium containing sorafenib for 12, 24, 48 and 72 h. The MTT assay was used to study the cytotoxic effects of sorafenib. Cell invasion and migration were assayed in wound healing and transwell experiments, respectively. Protein expression levels were evaluated by western blotting. The results show that sorafenib treatment decreased cell viability in a dose- and time-dependent manner. Sorafenib inhibited cell migration and invasion and decreased the expression of MMP-2 and MMP-9. Moreover, addition of the recombinant plasmid pCI-neo/ CREB (PN) reversed the sorafenib-induced inhibition of cell proliferation, migration and invasion. These results show that CREB is associated with the sorafenib-induced inhibition of proliferation, migration and invasion.https://doi.org/10.2478/acph-2018-0033crebrenal cancersorafenibmigrationinvasion
collection DOAJ
language English
format Article
sources DOAJ
author Huang Shuaishuai
Cui Pinger
Lin Shuangxia
Yao Xuping
Wang Xue
Ren Yu
Weng Guobin
spellingShingle Huang Shuaishuai
Cui Pinger
Lin Shuangxia
Yao Xuping
Wang Xue
Ren Yu
Weng Guobin
The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion
Acta Pharmaceutica
creb
renal cancer
sorafenib
migration
invasion
author_facet Huang Shuaishuai
Cui Pinger
Lin Shuangxia
Yao Xuping
Wang Xue
Ren Yu
Weng Guobin
author_sort Huang Shuaishuai
title The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion
title_short The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion
title_full The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion
title_fullStr The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion
title_full_unstemmed The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion
title_sort transcription factor creb is involved in sorafenib-inhibited renal cancer cell proliferation, migration and invasion
publisher Sciendo
series Acta Pharmaceutica
issn 1846-9558
publishDate 2018-12-01
description Our previous reports showed that the cyclic-AMP-response element-binding protein (CREB) served as a proto-oncogene in the process of tumorigenesis and mediated the growth and metastatic activity of renal cancer cells. Our study, therefore, explored the role of CREB in sorafenib- -inhibited cell proliferation, migration and invasion. Renal cancer cells were cultured in medium containing sorafenib for 12, 24, 48 and 72 h. The MTT assay was used to study the cytotoxic effects of sorafenib. Cell invasion and migration were assayed in wound healing and transwell experiments, respectively. Protein expression levels were evaluated by western blotting. The results show that sorafenib treatment decreased cell viability in a dose- and time-dependent manner. Sorafenib inhibited cell migration and invasion and decreased the expression of MMP-2 and MMP-9. Moreover, addition of the recombinant plasmid pCI-neo/ CREB (PN) reversed the sorafenib-induced inhibition of cell proliferation, migration and invasion. These results show that CREB is associated with the sorafenib-induced inhibition of proliferation, migration and invasion.
topic creb
renal cancer
sorafenib
migration
invasion
url https://doi.org/10.2478/acph-2018-0033
work_keys_str_mv AT huangshuaishuai thetranscriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT cuipinger thetranscriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT linshuangxia thetranscriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT yaoxuping thetranscriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT wangxue thetranscriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT renyu thetranscriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT wengguobin thetranscriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT huangshuaishuai transcriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT cuipinger transcriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT linshuangxia transcriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT yaoxuping transcriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT wangxue transcriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT renyu transcriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
AT wengguobin transcriptionfactorcrebisinvolvedinsorafenibinhibitedrenalcancercellproliferationmigrationandinvasion
_version_ 1717767307737956352

Similar Items