Effects of P22 bacteriophage on salmonella Enterica subsp. enterica serovar Typhimurium DMC4 strain biofilm formation and eradication

• Main Authors: Karaca Basar, Akcelik Nefise, Akcelik Mustafa
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2015-01-01
• Series: Archives of Biological Sciences
• Subjects: Salmonella; biofilm; bacterial adhesion; bacteriophage; eradication
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500114K.pdf

Over the last decades, several antimicrobial agents have been made available. Due to increasing antimicrobial resistance, bacteriophages were rediscovered for their potential applications against bacterial infections. In the present study, biofilm inhibition and eradication of Salmonella enterica subsp. enterica serovar Typhimurium DMC4 strain (S. Typhimurium) was evaluated with respect to different incubation periods at different P22 phage titrations. The efficacy of P22 phage on biofilm formation and eradication of S. Typhimurium DMC4 strain was screened in vitro on polystyrene and stainless steel surfaces. The biofilm forming capacity of S. Typhimurium was significantly reduced at higher phage titrations (106 pfu/mL ≤). All phage titers (104-108 pfu/mL) were found to be effective at the end of the 24 h-incubation period whereas higher phage titrations were found to be effective at the end of the 48 h and 72 h of incubation. P22 phage has less efficacy on already formed, especially mature biofilms (72 h-old biofilm). Notable results of P22 phage treatment on S. Typhimurium biofilm suggest that P22 phage has potential uses in food systems.