Rapid Colorimetric Assay for Detection of Listeria monocytogenes in Food Samples Using LAMP Formation of DNA Concatemers and Gold Nanoparticle-DNA Probe Complex

Rapid Colorimetric Assay for Detection of Listeria monocytogenes in Food Samples Using LAMP Formation of DNA Concatemers and Gold Nanoparticle-DNA Probe Complex

Listeria monocytogenes is a major foodborne pathogen of global health concern. Herein, the rapid diagnosis of L. monocytogenes has been achieved using loop-mediated isothermal amplification (LAMP) based on the phosphatidylcholine-phospholipase C gene (plcB). Colorimetric detection was then performed...

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Bibliographic Details
Main Authors: Sirirat Wachiralurpan, Thayat Sriyapai, Supatra Areekit, Pichapak Sriyapai, Suphitcha Augkarawaritsawong, Somchai Santiwatanakul, Kosum Chansiri
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-04-01
Series:Frontiers in Chemistry
Subjects:
L. monocytogenes
plcB gene
loop-mediated isothermal amplification (LAMP)
gold nanoparticle/DNA probe
rapid colorimetric detection
Online Access:http://journal.frontiersin.org/article/10.3389/fchem.2018.00090/full

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