Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer

Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer

Summary: Background/Objective: Positive carcinoembryonic antigen (CEA) messenger RNA (mRNA) in peritoneal lavage is associated with poor prognosis in patients with colon cancer. However, there are no reports about rectal cancer. Therefore we aimed to evaluate the frequency of positive CEA mRNA in p...

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Main Authors: Koji Murono, Soichiro Ishihara, Kazushige Kawai, Manabu Kaneko, Kazuhito Sasaki, Kensuke Otani, Koji Yasuda, Takeshi Nishikawa, Toshiaki Tanaka, Tomomichi Kiyomatsu, Keisuke Hata, Hiroaki Nozawa, Yumiko Satoh, Makiko Kurihara, Yutaka Yatomi, Toshiaki Watanabe
Format: Article
Language:English
Published: Elsevier 2018-07-01
Series:Asian Journal of Surgery
Online Access:http://www.sciencedirect.com/science/article/pii/S1015958416303761
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spelling doaj-4431aac4d91b4744a1d54f2fd52719192020-11-24T21:07:48ZengElsevierAsian Journal of Surgery1015-95842018-07-01414321327Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancerKoji Murono0Soichiro Ishihara1Kazushige Kawai2Manabu Kaneko3Kazuhito Sasaki4Kensuke Otani5Koji Yasuda6Takeshi Nishikawa7Toshiaki Tanaka8Tomomichi Kiyomatsu9Keisuke Hata10Hiroaki Nozawa11Yumiko Satoh12Makiko Kurihara13Yutaka Yatomi14Toshiaki Watanabe15Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan; Corresponding author. Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, JapanDivision of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanSummary: Background/Objective: Positive carcinoembryonic antigen (CEA) messenger RNA (mRNA) in peritoneal lavage is associated with poor prognosis in patients with colon cancer. However, there are no reports about rectal cancer. Therefore we aimed to evaluate the frequency of positive CEA mRNA in peritoneal lavage and the significance of CEA mRNA in patients with low rectal cancer. Methods: A total of 55 patients with low rectal cancer who received curative surgical resection were enrolled. CEA mRNA in peritoneal lavage was measured using the transcription–reverse transcription concerted method, a quantitative RNA amplification method. The correlation between CEA mRNA and overall and peritoneal recurrence-free survival was evaluated. Results: Among 55 patients, 6 (10.9%) had positive CEA mRNA in peritoneal lavage. Patients with positive CEA mRNA resulted in significantly higher recurrence rate than those with negative CEA mRNA (p=0.007). Similarly, the local recurrence rate was significantly higher in the positive CEA mRNA group than in the negative CEA mRNA group (p=0.0009). Lymph node metastasis and positive CEA mRNA were independent risk factors for overall and local recurrence. Conclusion: Positive CEA mRNA in low rectal cancer is a factor that predisposes patients to a high risk for overall recurrence, especially for local recurrence. Keywords: carcinoembryonic antigen messenger RNA, low rectal cancer, micrometastasis, peritoneal lavage, transcription–reverse transcription concerted methodhttp://www.sciencedirect.com/science/article/pii/S1015958416303761
collection DOAJ
language English
format Article
sources DOAJ
author Koji Murono
Soichiro Ishihara
Kazushige Kawai
Manabu Kaneko
Kazuhito Sasaki
Kensuke Otani
Koji Yasuda
Takeshi Nishikawa
Toshiaki Tanaka
Tomomichi Kiyomatsu
Keisuke Hata
Hiroaki Nozawa
Yumiko Satoh
Makiko Kurihara
Yutaka Yatomi
Toshiaki Watanabe
spellingShingle Koji Murono
Soichiro Ishihara
Kazushige Kawai
Manabu Kaneko
Kazuhito Sasaki
Kensuke Otani
Koji Yasuda
Takeshi Nishikawa
Toshiaki Tanaka
Tomomichi Kiyomatsu
Keisuke Hata
Hiroaki Nozawa
Yumiko Satoh
Makiko Kurihara
Yutaka Yatomi
Toshiaki Watanabe
Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer
Asian Journal of Surgery
author_facet Koji Murono
Soichiro Ishihara
Kazushige Kawai
Manabu Kaneko
Kazuhito Sasaki
Kensuke Otani
Koji Yasuda
Takeshi Nishikawa
Toshiaki Tanaka
Tomomichi Kiyomatsu
Keisuke Hata
Hiroaki Nozawa
Yumiko Satoh
Makiko Kurihara
Yutaka Yatomi
Toshiaki Watanabe
author_sort Koji Murono
title Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer
title_short Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer
title_full Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer
title_fullStr Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer
title_full_unstemmed Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer
title_sort significance of carcinoembryonic antigen mrna in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer
publisher Elsevier
series Asian Journal of Surgery
issn 1015-9584
publishDate 2018-07-01
description Summary: Background/Objective: Positive carcinoembryonic antigen (CEA) messenger RNA (mRNA) in peritoneal lavage is associated with poor prognosis in patients with colon cancer. However, there are no reports about rectal cancer. Therefore we aimed to evaluate the frequency of positive CEA mRNA in peritoneal lavage and the significance of CEA mRNA in patients with low rectal cancer. Methods: A total of 55 patients with low rectal cancer who received curative surgical resection were enrolled. CEA mRNA in peritoneal lavage was measured using the transcription–reverse transcription concerted method, a quantitative RNA amplification method. The correlation between CEA mRNA and overall and peritoneal recurrence-free survival was evaluated. Results: Among 55 patients, 6 (10.9%) had positive CEA mRNA in peritoneal lavage. Patients with positive CEA mRNA resulted in significantly higher recurrence rate than those with negative CEA mRNA (p=0.007). Similarly, the local recurrence rate was significantly higher in the positive CEA mRNA group than in the negative CEA mRNA group (p=0.0009). Lymph node metastasis and positive CEA mRNA were independent risk factors for overall and local recurrence. Conclusion: Positive CEA mRNA in low rectal cancer is a factor that predisposes patients to a high risk for overall recurrence, especially for local recurrence. Keywords: carcinoembryonic antigen messenger RNA, low rectal cancer, micrometastasis, peritoneal lavage, transcription–reverse transcription concerted method
url http://www.sciencedirect.com/science/article/pii/S1015958416303761
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