Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from <it>Pichia pastoris</it> using response surface methodology

Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from <it>Pichia pastoris</it> using response surface methodology

<p>Abstract</p> <p>Background</p> <p>Cell disruption strategies by high pressure homogenizer for the release of recombinant Hepatitis B surface antigen (HBsAg) from <it>Pichia pastoris</it> expression cells were optimized using response surface methodology (...

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Bibliographic Details
Main Authors: Tam Yew, Allaudin Zeenathul, Lila Mohd Azmi, Bahaman Abdul, Tan Joo, Rezaei Morvarid
Format: Article
Language:English
Published: BMC 2012-10-01
Series:BMC Biotechnology
Subjects:
Hepatitis B surface antigen
Cell disruption
Glass bead
High pressure homogenizer
<it>Pichia pastoris</it>
Recombinant protein
Online Access:http://www.biomedcentral.com/1472-6750/12/70

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