The effect of reverse transcription enzymes and conditions on high throughput amplicon sequencing of the 16S rRNA
It is assumed that the sequencing of ribosomes better reflects the active microbial community than the sequencing of the ribosomal RNA encoding genes. Yet, many studies exploring microbial communities in various environments, ranging from the human gut to deep oceans, questioned the validity of this...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2019-10-01
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| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/7608.pdf |