Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)

Snake fruit (Salacca zalacca (Gaert.) Voss) is a fruit species traditionally cultivated in Indonesia and other Southeast Asian countries. The edible parts of the fruits contain a certain amount of total phenolic, flavonoid, and monoterpenoid compounds, proving them to be their perfect sources. The m...

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Main Authors: Petra Hlásná Čepková, Michal Jágr, Dagmar Janovská, Václav Dvořáček, Anna Kotrbová Kozak, Iva Viehmannová
Format: Article
Language:English
Published: Hindawi-Wiley 2021-01-01
Series:Journal of Food Quality
Online Access:http://dx.doi.org/10.1155/2021/6621811
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spelling doaj-440eac1f04314823a2977ea1b1eb43fb2021-04-05T00:00:31ZengHindawi-WileyJournal of Food Quality1745-45572021-01-01202110.1155/2021/6621811Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)Petra Hlásná Čepková0Michal Jágr1Dagmar Janovská2Václav Dvořáček3Anna Kotrbová Kozak4Iva Viehmannová5Gene BankQuality of Plant ProductsGene BankQuality of Plant ProductsQuality of Plant ProductsDepartment of Crop Sciences and AgroforestrySnake fruit (Salacca zalacca (Gaert.) Voss) is a fruit species traditionally cultivated in Indonesia and other Southeast Asian countries. The edible parts of the fruits contain a certain amount of total phenolic, flavonoid, and monoterpenoid compounds, proving them to be their perfect sources. The main goal of this work was to detect, quantify, and identify various phenolic compounds present in snake fruit pulp. Ultrahigh performance liquid chromatography coupled to a Q-Orbitrap tandem mass spectrometer was able to detect 19 phenolic compounds in the salak pulp, including 5 flavanols, 6 phenolic acids, 2 flavonols, 1 flavone, and also 5 presumably new phenolic compounds. Among the detected compounds, 11 were reported and quantified for the first time in salak pulp. Chlorogenic acid was by far the most predominant phenolic compound. The next relatively abundant compounds in snake fruit were epicatechin, isoquercetin, neochlorogenic acid, ferulic acid, gallic acid and procyanidine B2 (levels at ca 5–10 μg/g in MeOH extract), syringic acid, and caffeic acid (levels at ca 1 μg/g in H2O extract). A significant total phenolic content (257.17 μL/mL) and antioxidant activities (10.56 μM TE/g of fruit pulp) were determined. In conclusion, S. zalacca fruit has potential to serve as a natural source of phenolic compounds with antioxidative activities which may be associated with their health benefits.http://dx.doi.org/10.1155/2021/6621811
collection DOAJ
language English
format Article
sources DOAJ
author Petra Hlásná Čepková
Michal Jágr
Dagmar Janovská
Václav Dvořáček
Anna Kotrbová Kozak
Iva Viehmannová
spellingShingle Petra Hlásná Čepková
Michal Jágr
Dagmar Janovská
Václav Dvořáček
Anna Kotrbová Kozak
Iva Viehmannová
Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)
Journal of Food Quality
author_facet Petra Hlásná Čepková
Michal Jágr
Dagmar Janovská
Václav Dvořáček
Anna Kotrbová Kozak
Iva Viehmannová
author_sort Petra Hlásná Čepková
title Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)
title_short Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)
title_full Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)
title_fullStr Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)
title_full_unstemmed Comprehensive Mass Spectrometric Analysis of Snake Fruit: Salak (Salacca zalacca)
title_sort comprehensive mass spectrometric analysis of snake fruit: salak (salacca zalacca)
publisher Hindawi-Wiley
series Journal of Food Quality
issn 1745-4557
publishDate 2021-01-01
description Snake fruit (Salacca zalacca (Gaert.) Voss) is a fruit species traditionally cultivated in Indonesia and other Southeast Asian countries. The edible parts of the fruits contain a certain amount of total phenolic, flavonoid, and monoterpenoid compounds, proving them to be their perfect sources. The main goal of this work was to detect, quantify, and identify various phenolic compounds present in snake fruit pulp. Ultrahigh performance liquid chromatography coupled to a Q-Orbitrap tandem mass spectrometer was able to detect 19 phenolic compounds in the salak pulp, including 5 flavanols, 6 phenolic acids, 2 flavonols, 1 flavone, and also 5 presumably new phenolic compounds. Among the detected compounds, 11 were reported and quantified for the first time in salak pulp. Chlorogenic acid was by far the most predominant phenolic compound. The next relatively abundant compounds in snake fruit were epicatechin, isoquercetin, neochlorogenic acid, ferulic acid, gallic acid and procyanidine B2 (levels at ca 5–10 μg/g in MeOH extract), syringic acid, and caffeic acid (levels at ca 1 μg/g in H2O extract). A significant total phenolic content (257.17 μL/mL) and antioxidant activities (10.56 μM TE/g of fruit pulp) were determined. In conclusion, S. zalacca fruit has potential to serve as a natural source of phenolic compounds with antioxidative activities which may be associated with their health benefits.
url http://dx.doi.org/10.1155/2021/6621811
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