Durable CRISPR-Based Epigenetic Silencing

• Main Authors: Muneaki Nakamura, Alexis E. Ivec, Yuchen Gao, Lei S. Qi
• Format: Article
• Language: English
• Published: American Association for the Advancement of Science 2021-01-01
• Series: BioDesign Research
• Online Access: http://dx.doi.org/10.34133/2021/9815820

Development of CRISPR-based epigenome editing tools is important for the study and engineering of biological behavior. Here, we describe the design of a reporter system for quantifying the ability of CRISPR epigenome editors to produce a stable gene repression. We characterize the dynamics of durable gene silencing and reactivation, as well as the induced epigenetic changes of this system. We report the creation of single-protein CRISPR constructs bearing combinations of three epigenetic editing domains, termed KAL, that can stably repress the gene expression. This system should allow for the development of novel epigenome editing tools which will be useful in a wide array of biological research and engineering applications.