Validation of a “clearing” assay for milk lipoprotein lipase in agarose gel

We have developed a simplified method for the quantitative assay of lipoprotein lipase in cow's milk based on the hydrolysis of a glyceride emulsion in semisolid agarose gel. The area of clearing produced thereby is a function of enzyme concentration. Absolute molar rates for unknown samples ma...

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Bibliographic Details
Main Authors: Dana E. Wilson, Carolyn M. Flowers, James C. Reading
Format: Article
Language:English
Published: Elsevier 1973-01-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752039338X