Studies on the Sorption Behaviour of Some Amino Acids on Silica Gel Pretreated with Alkalies in Relation to Chromatography
Silica gel for chromatography has been subjected to chemical pretreatment with aqueous NaOH and KOH solutions. The surface-phase pH, surface area, Na+ and K+ ion content and H+-ion exchange capacity of the samples have been determined. Sorption–desorption studies of the amino acids, l (+)-arginine a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi - SAGE Publishing
1991-09-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1177/026361749100800305 |
| Summary: | Silica gel for chromatography has been subjected to chemical pretreatment with aqueous NaOH and KOH solutions. The surface-phase pH, surface area, Na+ and K+ ion content and H+-ion exchange capacity of the samples have been determined. Sorption–desorption studies of the amino acids, l (+)-arginine and l (–)-histidine, were carried out from 90% ethanol solution on substrates whose surface-phase pH was in the range 7.0–11.5. Sorption was found to be pH-dependent, with maximum sorption occurring over the pH range 7.5–10.0. The process was fast (10 min to 24 h) and almost athermic (20–50°C). The desorption efficiency and retarding influence of metallic cations on the adsorption were in the order: Th4+ > Al3+ > Mg2+ > Na+. Elution studies and column chromatography of a few synthetic amino acid mixtures have also been carried out. |
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| ISSN: | 0263-6174 2048-4038 |