Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application

• Main Authors: Dong Y, Zhuang H, Hao Y, Zhang L, Yang Q, Liu Y, Qi C, Wang S
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-03-01
• Series: International Journal of Nanomedicine
• Subjects: thermo-sensitive hydrogels; wound dressing; superoxide dismutase; poly (γ-glutamic acid)
• Online Access: https://www.dovepress.com/polyn-isopropyl-acrylamidepolygamma-glutamic-acid-thermo-sensitive-hyd-peer-reviewed-article-IJN

Yunsheng Dong,1 Huahong Zhuang,1 Yan Hao,1 Lin Zhang,1 Qiang Yang,2 Yufei Liu,1 Chunxiao Qi,1 Shufang Wang1 1State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, The College of Life Science, Nankai University, Tianjin, People’s Republic of China; 2Department of Spine Surgery, Tianjin Hospital, Tianjin, People’s Republic of ChinaCorrespondence: Qiang Yang No. 406 Jiefang Nan Road, Tianjin 300211, People’s Republic of ChinaEmail [e-mail] Wang No. 94 Weijin Road, Nankai District, Tianjin 300071, People’s Republic of ChinaEmail [e-mail]: Chronic trauma repair is an important issue affecting people’s healthy lives. Thermo-sensitive hydrogel is injectable in situ and can be used to treat large-area wounds. In addition, antioxidants play important roles in promoting wound repair.Methods: The purpose of this research was to prepare a novel thermo-sensitive hydrogel-poly(N-isopropyl-acrylamide)/poly(γ-glutamic acid) (PP) loaded with superoxide dismutase (SOD) to improve the effect for trauma treatment. The micromorphology of the hydrogel was observed by scanning electron microscope and the physical properties were measured. The biocompatibility of hydrogel was evaluated by MTT experiment, and the effect of hydrogel on skin wound healing was evaluated by in vivo histological staining.Results: Gelling behavior and differential scanning calorimeter outcomes showed that the PP hydrogels possessed thermo-sensitivity at physiological temperature and the phase transformation temperature was 28.2°C. The high swelling rate and good water retention were conducive to wound healing. The activity of SOD in vitro was up to 85% at 10 h, which was advantageous to eliminate the superoxide anion. MTT assay revealed that this hydrogel possessed good biocompatibility. Dressings of PP loaded with SOD (SOD-PP) had a higher wound closure rate than other treatments in vivo in diabetic rat model.Discussion: The SOD-PP thermo-sensitive hydrogels can effectively promote wound healing and have good application prospects for wound repair.Keywords: thermo-sensitive hydrogels, wound dressing, superoxide dismutase, poly(γ-glutamic acid)