Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A

Olivieri, Walker, Karamafrooz et al. show that the fusion of the dynamic J-domain to PKA-C (PKA-CDNAJB1) disrupts the internal allosteric network, attenuating the nucleotide/PKI binding cooperativity. This study suggests that the reduced allosteric cooperativity may contribute to the pathology that...

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Main Authors:	Cristina Olivieri, Caitlin Walker, Adak Karamafrooz, Yingjie Wang, V. S. Manu, Fernando Porcelli, Donald K. Blumenthal, David D. Thomas, David A. Bernlohr, Simon M. Sandford, Susan S. Taylor, Gianluigi Veglia
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-03-01
Series:	Communications Biology
Online Access:	https://doi.org/10.1038/s42003-021-01819-6

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spelling	doaj-43c1fcd3a0854b83a040ab4779192c9e2021-03-11T12:04:34ZengNature Publishing GroupCommunications Biology2399-36422021-03-014111110.1038/s42003-021-01819-6Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase ACristina Olivieri0Caitlin Walker1Adak Karamafrooz2Yingjie Wang3V. S. Manu4Fernando Porcelli5Donald K. Blumenthal6David D. Thomas7David A. Bernlohr8Simon M. Sandford9Susan S. Taylor10Gianluigi Veglia11Department of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDIBAF - University of Tuscia – Largo dell’ UniversitàDepartment of Pharmacology and Toxicology, University of UtahDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaLaboratory of Cellular Biophysics, Rockefeller UniversityDepartment of Chemistry and Biochemistry and Pharmacology, University of California at San DiegoDepartment of Biochemistry, Molecular Biology, and Biophysics, University of MinnesotaOlivieri, Walker, Karamafrooz et al. show that the fusion of the dynamic J-domain to PKA-C (PKA-CDNAJB1) disrupts the internal allosteric network, attenuating the nucleotide/PKI binding cooperativity. This study suggests that the reduced allosteric cooperativity may contribute to the pathology that PKA-CDNAJB1 drives.https://doi.org/10.1038/s42003-021-01819-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Cristina Olivieri Caitlin Walker Adak Karamafrooz Yingjie Wang V. S. Manu Fernando Porcelli Donald K. Blumenthal David D. Thomas David A. Bernlohr Simon M. Sandford Susan S. Taylor Gianluigi Veglia
spellingShingle	Cristina Olivieri Caitlin Walker Adak Karamafrooz Yingjie Wang V. S. Manu Fernando Porcelli Donald K. Blumenthal David D. Thomas David A. Bernlohr Simon M. Sandford Susan S. Taylor Gianluigi Veglia Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A Communications Biology
author_facet	Cristina Olivieri Caitlin Walker Adak Karamafrooz Yingjie Wang V. S. Manu Fernando Porcelli Donald K. Blumenthal David D. Thomas David A. Bernlohr Simon M. Sandford Susan S. Taylor Gianluigi Veglia
author_sort	Cristina Olivieri
title	Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A
title_short	Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A
title_full	Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A
title_fullStr	Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A
title_full_unstemmed	Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A
title_sort	defective internal allosteric network imparts dysfunctional atp/substrate-binding cooperativity in oncogenic chimera of protein kinase a
publisher	Nature Publishing Group
series	Communications Biology
issn	2399-3642
publishDate	2021-03-01
description	Olivieri, Walker, Karamafrooz et al. show that the fusion of the dynamic J-domain to PKA-C (PKA-CDNAJB1) disrupts the internal allosteric network, attenuating the nucleotide/PKI binding cooperativity. This study suggests that the reduced allosteric cooperativity may contribute to the pathology that PKA-CDNAJB1 drives.
url	https://doi.org/10.1038/s42003-021-01819-6
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Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A

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