The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines

The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines

The effects of an antioxidative pentapeptide from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines were investigated. The bioactive pentapeptide had an amino acid sequence of Asn–Arg–Try–His–Glu (NRYHE). Caco-2 and HT-29 cells were pretreated with peptide (0.05, 0.1,...

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Main Authors: Yujie Guo, Tao Zhang, Bo Jiang, Ming Miao, Wanmeng Mu
Format: Article
Language:English
Published: Elsevier 2014-03-01
Series:Journal of Functional Foods
Subjects:
Bioactive peptide
Caco-2
HT-29
Antioxidant enzymes
Nrf2
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464613003071
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spelling doaj-43ab7b677ca144459f2ba1d3c43ae8b72021-04-29T04:42:00ZengElsevierJournal of Functional Foods1756-46462014-03-017719726The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell linesYujie Guo0Tao Zhang1Bo Jiang2Ming Miao3Wanmeng Mu4State Key Laboratory of Food Science and Technology, Ministry of Education, Key Laboratory of Carbohydrate Chemistry & Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaCorresponding author. Tel./fax: 86 510 85919161.; State Key Laboratory of Food Science and Technology, Ministry of Education, Key Laboratory of Carbohydrate Chemistry & Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Food Science and Technology, Ministry of Education, Key Laboratory of Carbohydrate Chemistry & Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Food Science and Technology, Ministry of Education, Key Laboratory of Carbohydrate Chemistry & Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Food Science and Technology, Ministry of Education, Key Laboratory of Carbohydrate Chemistry & Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaThe effects of an antioxidative pentapeptide from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines were investigated. The bioactive pentapeptide had an amino acid sequence of Asn–Arg–Try–His–Glu (NRYHE). Caco-2 and HT-29 cells were pretreated with peptide (0.05, 0.1, 0.25 and 0.5 mg/mL) for 2 h and stimulated with 1 mM H2O2 for 6 h. The activity of three important antioxidative enzymes, catalase, glutathione reductase and glutathione peroxidase, increased in a concentration-dependent manner. It was observed that peptide treatment elevated the expression of Nrf2 mRNA and several relative genes NQO1, HO-1, γ-GCS regulated by Nrf2 compared to the positive control. The trends in HT-29 and Caco-2 cells were similar.http://www.sciencedirect.com/science/article/pii/S1756464613003071Bioactive peptideCaco-2HT-29Antioxidant enzymesNrf2
collection DOAJ
language English
format Article
sources DOAJ
author Yujie Guo
Tao Zhang
Bo Jiang
Ming Miao
Wanmeng Mu
spellingShingle Yujie Guo
Tao Zhang
Bo Jiang
Ming Miao
Wanmeng Mu
The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines
Journal of Functional Foods
Bioactive peptide
Caco-2
HT-29
Antioxidant enzymes
Nrf2
author_facet Yujie Guo
Tao Zhang
Bo Jiang
Ming Miao
Wanmeng Mu
author_sort Yujie Guo
title The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines
title_short The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines
title_full The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines
title_fullStr The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines
title_full_unstemmed The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines
title_sort effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in caco-2 and ht-29 cell lines
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2014-03-01
description The effects of an antioxidative pentapeptide from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines were investigated. The bioactive pentapeptide had an amino acid sequence of Asn–Arg–Try–His–Glu (NRYHE). Caco-2 and HT-29 cells were pretreated with peptide (0.05, 0.1, 0.25 and 0.5 mg/mL) for 2 h and stimulated with 1 mM H2O2 for 6 h. The activity of three important antioxidative enzymes, catalase, glutathione reductase and glutathione peroxidase, increased in a concentration-dependent manner. It was observed that peptide treatment elevated the expression of Nrf2 mRNA and several relative genes NQO1, HO-1, γ-GCS regulated by Nrf2 compared to the positive control. The trends in HT-29 and Caco-2 cells were similar.
topic Bioactive peptide
Caco-2
HT-29
Antioxidant enzymes
Nrf2
url http://www.sciencedirect.com/science/article/pii/S1756464613003071
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