The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines

The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines

The effects of an antioxidative pentapeptide from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines were investigated. The bioactive pentapeptide had an amino acid sequence of Asn–Arg–Try–His–Glu (NRYHE). Caco-2 and HT-29 cells were pretreated with peptide (0.05, 0.1,...

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Bibliographic Details
Main Authors: Yujie Guo, Tao Zhang, Bo Jiang, Ming Miao, Wanmeng Mu
Format: Article
Language:English
Published: Elsevier 2014-03-01
Series:Journal of Functional Foods
Subjects:
Bioactive peptide
Caco-2
HT-29
Antioxidant enzymes
Nrf2
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464613003071
Description
Summary:The effects of an antioxidative pentapeptide from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines were investigated. The bioactive pentapeptide had an amino acid sequence of Asn–Arg–Try–His–Glu (NRYHE). Caco-2 and HT-29 cells were pretreated with peptide (0.05, 0.1, 0.25 and 0.5 mg/mL) for 2 h and stimulated with 1 mM H2O2 for 6 h. The activity of three important antioxidative enzymes, catalase, glutathione reductase and glutathione peroxidase, increased in a concentration-dependent manner. It was observed that peptide treatment elevated the expression of Nrf2 mRNA and several relative genes NQO1, HO-1, γ-GCS regulated by Nrf2 compared to the positive control. The trends in HT-29 and Caco-2 cells were similar.
ISSN:1756-4646

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