Pyrosequencing for mini-barcoding of fresh and old museum specimens.

DNA barcoding is an effective approach for species identification and for discovery of new and/or cryptic species. Sanger sequencing technology is the method of choice for obtaining standard 650 bp cytochrome c oxidase subunit I (COI) barcodes. However, DNA degradation/fragmentation makes it difficu...

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Bibliographic Details
Main Authors: Shadi Shokralla, Xin Zhou, Daniel H Janzen, Winnie Hallwachs, Jean-François Landry, Luke M Jacobus, Mehrdad Hajibabaei
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3144868?pdf=render

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