Pyrosequencing for mini-barcoding of fresh and old museum specimens.
DNA barcoding is an effective approach for species identification and for discovery of new and/or cryptic species. Sanger sequencing technology is the method of choice for obtaining standard 650 bp cytochrome c oxidase subunit I (COI) barcodes. However, DNA degradation/fragmentation makes it difficu...
Main Authors: | Shadi Shokralla, Xin Zhou, Daniel H Janzen, Winnie Hallwachs, Jean-François Landry, Luke M Jacobus, Mehrdad Hajibabaei |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3144868?pdf=render |
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