SIMULTANEOUS DETERMINATION OF PROTOCATECHUIC ALDEHYDE AND PROTOCATECHUIC ACID USING THE LOCALIZED SURFACE PLASMON RESONANCE PEAK OF SILVER NANOPARTICLES AND CHEMOMETRIC METHODS

• Main Authors: Javad Khodaveisi, Ali Mohammad Haji Shabani, Shayessteh Dadfarnia, Masoud Rohani Moghadam, Mohammad Reza Hormozi-Nezhad
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2015-08-01
• Series: Química Nova
• Subjects: protocatechuic aldehyde; protocatechuic acid; chemometric methods
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000700896&lng=en&tlng=en

A simple and sensitive spectrophotometric method is proposed for the simultaneous determination of protocatechuic acid and protocatechuic aldehyde. The method is based on the difference in the kinetic rates of the reactions of analytes with [Ag(NH3)2]+ in the presence of polyvinylpyrrolidone to produce silver nanoparticles. The data obtained were processed by chemometric methods using principal component analysis artificial neural network and partial least squares. Excellent linearity was obtained in the concentration ranges of 1.23-58.56 µg mL-1 and 0.08-30.39 µg mL-1 for PAC and PAH, respectively. The limits of detection for PAC and PAH were 0.039 and 0.025 µg mL-1, respectively.