Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats

Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats

The presence of Mycoplasma haemomuris (haemoplasma) in blood samples collected from specific pathogen-free (SPF) laboratory rats bred in Japan was reported. Its presence was examined in Fischer 344, Sprague-Dawley (SD), and Wistar rat strains of both sexes by real-time PCR. All strains were positive...

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Main Authors: Sashida Hinako, Harasawa Ryô, Ichijo Toshihiro, Satoh Hiroshi, Furuhama Kazuhisa
Format: Article
Language:English
Published: Sciendo 2014-09-01
Series:Bulletin of the Veterinary Institute in Pulawy
Subjects:
rat
specific pathogen free state
mycoplasma haemomuris
Online Access:https://doi.org/10.2478/bvip-2014-0058
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spelling doaj-4346181af0954d06bc18b06ebec82b722021-09-22T06:13:24ZengSciendoBulletin of the Veterinary Institute in Pulawy2300-32352014-09-0158337537810.2478/bvip-2014-0058bvip-2014-0058Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free RatsSashida Hinako0Harasawa Ryô1Ichijo Toshihiro2Satoh Hiroshi3Furuhama Kazuhisa4Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, JapanCooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, JapanCooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, JapanCooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, JapanCooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, JapanThe presence of Mycoplasma haemomuris (haemoplasma) in blood samples collected from specific pathogen-free (SPF) laboratory rats bred in Japan was reported. Its presence was examined in Fischer 344, Sprague-Dawley (SD), and Wistar rat strains of both sexes by real-time PCR. All strains were positive for M. haemomuris infection. The 16S rRNA gene of M. haemomuris strain detected in the animals was amplified using end-point PCR. Only the entire nucleotide sequence of 16S rRNA gene of a mycoplasma strain detected in SD rats was determined and compared to those of other haemoplasmas. Our investigations suggest a wide M. haemomuris infection among the SPF rats purchased from commercial breeders in Japan.https://doi.org/10.2478/bvip-2014-0058ratspecific pathogen free statemycoplasma haemomuris
collection DOAJ
language English
format Article
sources DOAJ
author Sashida Hinako
Harasawa Ryô
Ichijo Toshihiro
Satoh Hiroshi
Furuhama Kazuhisa
spellingShingle Sashida Hinako
Harasawa Ryô
Ichijo Toshihiro
Satoh Hiroshi
Furuhama Kazuhisa
Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats
Bulletin of the Veterinary Institute in Pulawy
rat
specific pathogen free state
mycoplasma haemomuris
author_facet Sashida Hinako
Harasawa Ryô
Ichijo Toshihiro
Satoh Hiroshi
Furuhama Kazuhisa
author_sort Sashida Hinako
title Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats
title_short Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats
title_full Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats
title_fullStr Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats
title_full_unstemmed Haemotropic Mycoplasma Infection Revealed by Real-Time PCR in Specific Pathogen-Free Rats
title_sort haemotropic mycoplasma infection revealed by real-time pcr in specific pathogen-free rats
publisher Sciendo
series Bulletin of the Veterinary Institute in Pulawy
issn 2300-3235
publishDate 2014-09-01
description The presence of Mycoplasma haemomuris (haemoplasma) in blood samples collected from specific pathogen-free (SPF) laboratory rats bred in Japan was reported. Its presence was examined in Fischer 344, Sprague-Dawley (SD), and Wistar rat strains of both sexes by real-time PCR. All strains were positive for M. haemomuris infection. The 16S rRNA gene of M. haemomuris strain detected in the animals was amplified using end-point PCR. Only the entire nucleotide sequence of 16S rRNA gene of a mycoplasma strain detected in SD rats was determined and compared to those of other haemoplasmas. Our investigations suggest a wide M. haemomuris infection among the SPF rats purchased from commercial breeders in Japan.
topic rat
specific pathogen free state
mycoplasma haemomuris
url https://doi.org/10.2478/bvip-2014-0058
work_keys_str_mv AT sashidahinako haemotropicmycoplasmainfectionrevealedbyrealtimepcrinspecificpathogenfreerats
AT harasawaryo haemotropicmycoplasmainfectionrevealedbyrealtimepcrinspecificpathogenfreerats
AT ichijotoshihiro haemotropicmycoplasmainfectionrevealedbyrealtimepcrinspecificpathogenfreerats
AT satohhiroshi haemotropicmycoplasmainfectionrevealedbyrealtimepcrinspecificpathogenfreerats
AT furuhamakazuhisa haemotropicmycoplasmainfectionrevealedbyrealtimepcrinspecificpathogenfreerats
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