Magnetic separation and antibiotics selection enable enrichment of cells with ZFN/TALEN-induced mutations.

Magnetic separation and antibiotics selection enable enrichment of cells with ZFN/TALEN-induced mutations.

The ability to enrich cells with targeted mutations greatly facilitates the process of using engineered nucleases, including zinc-finger nucleases and transcription activator-like effector nucleases, to construct such cells. We previously used surrogate reporters to enrich cells containing nuclease-...

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Bibliographic Details
Main Authors: Hyojin Kim, Myung-Sun Kim, Gabbine Wee, Choong-il Lee, Hyongbum Kim, Jin-Soo Kim
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3575389?pdf=render

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