THE FACTORS OF CULTIVATION LIMITING CAROTENOGENESIS OF MUTANTS STREPTOMICES GLOBISPORUS 1912

• Main Authors: С. Л. Голембіовська, Л. В. Поліщук, А. Ю. Коцюк, Б. П. Мацелюх
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2016-03-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: стрептоміцети; каротиногенез; лімітуючі чинники
• Online Access: http://mbt.onu.edu.ua/article/view/65361

Aim. To set the limiting carotenogenic components of the synthetic medium and conditions of cultivation for mutant strains Streptomyces globisporus 1912 Нр7 and Нр7С. Methods. The synthetic agar media has been preparated with the pre-mathematically calculated ratio C:N. Influence of salts on the process of carotenoids biosynthesis has been determined by the method of holes, the aerial mycelium has been carried by the copies method. The sign of the carotenoids biosynthesis has been visual investigated. Results. The analysis of the unfavorable factors for carotenogenesis have been conducted for strain S. globisporus 4Lcр. On their basis the factors has been determined, which block carotenoids biosynthesis in mutant strains S. globisporus 1912 Нр7 and S. globisporus 1912 Нр7С. It is shown that 0,007% FeSO4 has inhibited the formation of carotenoids of tested strains on optimal medium Waxman and Krasylnikov CPI. It was shown that the content of carbon sources to nitrogen sources below 24:1 and pH below 7 without the use of CaCO3, that prevents acidification during cultivation has suppressed the carotenoids formation. Stimulation effect of stress factors, particularly MgSO4 on tested strains carotenogenesis has been established. Conclusions. There were installed the limiting carotenogenic cultivation factors for mutant strains S. globisporus 1912 and Нр7 Нр7С: 0.007% FeSO4, the ratio of C:N below 24:1, pH below 7, in the absence of CaCO3 and stimulating carotenogenesis salts, in particular МgЅО4.