Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum

Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum

While in search of an enzyme for the conversion of xylose to xylitol at elevated temperatures, a xylose reductase (XR) gene was identified in the genome of the thermophilic fungus Chaetomium thermophilum. The gene was heterologously expressed in Escherichia coli as a His6-tagged fusion protein and c...

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Bibliographic Details
Main Authors: Julian Quehenberger, Tom Reichenbach, Niklas Baumann, Lukas Rettenbacher, Christina Divne, Oliver Spadiut
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:International Journal of Molecular Sciences
Subjects:
xylose reductase
Chaetomium thermophilum
kinetics
structure
homology model
cofactor binding
stability
Online Access:http://www.mdpi.com/1422-0067/20/1/185

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