Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones

Abstract Background Helicobacter pylori susceptibility to clarithromycin and fluoroquinolone can be determined through Etest or molecular assays. We examined the correlation between phenotypic susceptibility (MIC results) and genotypic susceptibility in H. pylori strains isolated from gastric biopsi...

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Main Authors: Dana Binyamin, Nina Pastukh, Avi On, Maya Paritsky, Avi Peretz
Format: Article
Language:English
Published: BMC 2017-08-01
Series:Gut Pathogens
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13099-017-0198-5
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spelling doaj-430bd3b301d543c78c93fb7f1e56f2072020-11-24T23:29:37ZengBMCGut Pathogens1757-47492017-08-01911810.1186/s13099-017-0198-5Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolonesDana Binyamin0Nina Pastukh1Avi On2Maya Paritsky3Avi Peretz4Clinical Microbiology Laboratory Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan UniversityClinical Microbiology Laboratory Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan UniversityThe Faculty of Medicine in the Galilee, Bar Ilan UniversityThe Faculty of Medicine in the Galilee, Bar Ilan UniversityClinical Microbiology Laboratory Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan UniversityAbstract Background Helicobacter pylori susceptibility to clarithromycin and fluoroquinolone can be determined through Etest or molecular assays. We examined the correlation between phenotypic susceptibility (MIC results) and genotypic susceptibility in H. pylori strains isolated from gastric biopsies. Results Out of 85 duplicate biopsies obtained from patients from northern Israel who underwent endoscopy, 70 were positive for H. pylori using the CUTest (urease test). These biopsies were cultured for H. pylori and Etest breakpoints were determined for levofloxacin and clarithromycin. H. pylori detection, characterization of wild type (WT) or mutant alleles, and antibiotic resistance were determined molecularly using GenoType HelicoDR kit. Phenotypic and genotype results were compared. The molecular method had higher sensitivity for detection of H. pylori than culture (94.3% vs. 77.1%). Resistance to clarithromycin was higher than to levofloxacin by both methods. Levofloxacin resistance was found in 21.2 and 0% using genotypic and phenotypic methods, respectively. Clarithromycin resistance was found in 66.7 and 62.9% by genotypic and phenotypic methods, respectively. Some samples were found susceptible by Etest and resistant by GenoType HelicoDR (13/53 samples) and some vice versa (10/53 samples). Conclusion GenoType HelicoDR kit has high sensitivity rate for H. pylori detection. It has the ability to detect many mutations and can help to determine initial antibiotic treatment at the beginning of therapy. However, there were a few cases where there was a phenotype-genotype mismatch result that could derive from possible causes. For example, a resistance mechanism that is not tested in the kit.http://link.springer.com/article/10.1186/s13099-017-0198-5Helicobacter pyloriGenoType HelicoDR kitsClarithromycinLevofloxacin
collection DOAJ
language English
format Article
sources DOAJ
author Dana Binyamin
Nina Pastukh
Avi On
Maya Paritsky
Avi Peretz
spellingShingle Dana Binyamin
Nina Pastukh
Avi On
Maya Paritsky
Avi Peretz
Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones
Gut Pathogens
Helicobacter pylori
GenoType HelicoDR kits
Clarithromycin
Levofloxacin
author_facet Dana Binyamin
Nina Pastukh
Avi On
Maya Paritsky
Avi Peretz
author_sort Dana Binyamin
title Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones
title_short Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones
title_full Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones
title_fullStr Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones
title_full_unstemmed Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones
title_sort phenotypic and genotypic correlation as expressed in helicobacter pylori resistance to clarithromycin and fluoroquinolones
publisher BMC
series Gut Pathogens
issn 1757-4749
publishDate 2017-08-01
description Abstract Background Helicobacter pylori susceptibility to clarithromycin and fluoroquinolone can be determined through Etest or molecular assays. We examined the correlation between phenotypic susceptibility (MIC results) and genotypic susceptibility in H. pylori strains isolated from gastric biopsies. Results Out of 85 duplicate biopsies obtained from patients from northern Israel who underwent endoscopy, 70 were positive for H. pylori using the CUTest (urease test). These biopsies were cultured for H. pylori and Etest breakpoints were determined for levofloxacin and clarithromycin. H. pylori detection, characterization of wild type (WT) or mutant alleles, and antibiotic resistance were determined molecularly using GenoType HelicoDR kit. Phenotypic and genotype results were compared. The molecular method had higher sensitivity for detection of H. pylori than culture (94.3% vs. 77.1%). Resistance to clarithromycin was higher than to levofloxacin by both methods. Levofloxacin resistance was found in 21.2 and 0% using genotypic and phenotypic methods, respectively. Clarithromycin resistance was found in 66.7 and 62.9% by genotypic and phenotypic methods, respectively. Some samples were found susceptible by Etest and resistant by GenoType HelicoDR (13/53 samples) and some vice versa (10/53 samples). Conclusion GenoType HelicoDR kit has high sensitivity rate for H. pylori detection. It has the ability to detect many mutations and can help to determine initial antibiotic treatment at the beginning of therapy. However, there were a few cases where there was a phenotype-genotype mismatch result that could derive from possible causes. For example, a resistance mechanism that is not tested in the kit.
topic Helicobacter pylori
GenoType HelicoDR kits
Clarithromycin
Levofloxacin
url http://link.springer.com/article/10.1186/s13099-017-0198-5
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