Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system
Abstract Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay...
Main Authors: | Shigeo S. Sugano, Hiroko Suzuki, Eisuke Shimokita, Hirofumi Chiba, Sumihare Noji, Yuriko Osakabe, Keishi Osakabe |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-04-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-00883-5 |
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