Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells

Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells

The increasing number of multidrug-resistant microbes now emerging necessitates the identification of novel antimicrobial agents. Plants produce a great variety of antimicrobial peptides including hundreds of small, nodule-specific cysteine-rich NCR peptides that, in the legume Medicago truncatula,...

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Main Authors: Lilla Ördögh, Andrea Vörös, István Nagy, Éva Kondorosi, Attila Kereszt
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2014/320796
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spelling doaj-42d8cc229b0d459eac6fb46801691e812020-11-24T20:53:57ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/320796320796Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human CellsLilla Ördögh0Andrea Vörös1István Nagy2Éva Kondorosi3Attila Kereszt4Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari Korut 62, Szeged 6726, HungaryInstitute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari Korut 62, Szeged 6726, HungaryInstitute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari Korut 62, Szeged 6726, HungaryInstitute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari Korut 62, Szeged 6726, HungaryInstitute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari Korut 62, Szeged 6726, HungaryThe increasing number of multidrug-resistant microbes now emerging necessitates the identification of novel antimicrobial agents. Plants produce a great variety of antimicrobial peptides including hundreds of small, nodule-specific cysteine-rich NCR peptides that, in the legume Medicago truncatula, govern the differentiation of endosymbiotic nitrogen fixing bacteria and, in vitro, can display potent antibacterial activities. In this study, the potential candidacidal activity of 19 NCR peptides was investigated. Cationic NCR peptides having an isoelectric point above 9 were efficient in killing Candida albicans, one of the most common fungal pathogens of humans. None of the tested NCR peptides were toxic for immortalized human epithelial cells at concentrations that effectively killed the fungus; however, at higher concentrations, some of them inhibited the division of the cells. Furthermore, the cationic peptides successfully inhibited C. albicans induced human epithelial cell death in an in vitro coculture model. These results highlight the therapeutic potential of cationic NCR peptides in the treatment of candidiasis.http://dx.doi.org/10.1155/2014/320796
collection DOAJ
language English
format Article
sources DOAJ
author Lilla Ördögh
Andrea Vörös
István Nagy
Éva Kondorosi
Attila Kereszt
spellingShingle Lilla Ördögh
Andrea Vörös
István Nagy
Éva Kondorosi
Attila Kereszt
Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells
BioMed Research International
author_facet Lilla Ördögh
Andrea Vörös
István Nagy
Éva Kondorosi
Attila Kereszt
author_sort Lilla Ördögh
title Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells
title_short Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells
title_full Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells
title_fullStr Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells
title_full_unstemmed Symbiotic Plant Peptides Eliminate Candida albicans Both In Vitro and in an Epithelial Infection Model and Inhibit the Proliferation of Immortalized Human Cells
title_sort symbiotic plant peptides eliminate candida albicans both in vitro and in an epithelial infection model and inhibit the proliferation of immortalized human cells
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2014-01-01
description The increasing number of multidrug-resistant microbes now emerging necessitates the identification of novel antimicrobial agents. Plants produce a great variety of antimicrobial peptides including hundreds of small, nodule-specific cysteine-rich NCR peptides that, in the legume Medicago truncatula, govern the differentiation of endosymbiotic nitrogen fixing bacteria and, in vitro, can display potent antibacterial activities. In this study, the potential candidacidal activity of 19 NCR peptides was investigated. Cationic NCR peptides having an isoelectric point above 9 were efficient in killing Candida albicans, one of the most common fungal pathogens of humans. None of the tested NCR peptides were toxic for immortalized human epithelial cells at concentrations that effectively killed the fungus; however, at higher concentrations, some of them inhibited the division of the cells. Furthermore, the cationic peptides successfully inhibited C. albicans induced human epithelial cell death in an in vitro coculture model. These results highlight the therapeutic potential of cationic NCR peptides in the treatment of candidiasis.
url http://dx.doi.org/10.1155/2014/320796
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AT istvannagy symbioticplantpeptideseliminatecandidaalbicansbothinvitroandinanepithelialinfectionmodelandinhibittheproliferationofimmortalizedhumancells
AT evakondorosi symbioticplantpeptideseliminatecandidaalbicansbothinvitroandinanepithelialinfectionmodelandinhibittheproliferationofimmortalizedhumancells
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