Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier

The standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also...

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Main Author:	John Graham
Format:	Article
Language:	English
Published:	Hindawi Limited 2002-01-01
Series:	The Scientific World Journal
Online Access:	http://dx.doi.org/10.1100/tsw.2002.845

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spelling	doaj-42cb002df22b4df38b6581b7fdab9a542020-11-25T00:53:20ZengHindawi LimitedThe Scientific World Journal1537-744X2002-01-0121654165610.1100/tsw.2002.845Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density BarrierJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKThe standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also be isolated from whole blood by sedimentation on to a density barrier containing approx. 10% iodixanol, polysucrose (Ficoll) with a density of approx 1.074 g/ml.http://dx.doi.org/10.1100/tsw.2002.845
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	John Graham
spellingShingle	John Graham Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier The Scientific World Journal
author_facet	John Graham
author_sort	John Graham
title	Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_short	Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_full	Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_fullStr	Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_full_unstemmed	Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_sort	isolation of peripheral blood mononuclear cells from macaques on a density barrier
publisher	Hindawi Limited
series	The Scientific World Journal
issn	1537-744X
publishDate	2002-01-01
description	The standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also be isolated from whole blood by sedimentation on to a density barrier containing approx. 10% iodixanol, polysucrose (Ficoll) with a density of approx 1.074 g/ml.
url	http://dx.doi.org/10.1100/tsw.2002.845
work_keys_str_mv	AT johngraham isolationofperipheralbloodmononuclearcellsfrommacaquesonadensitybarrier
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Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier

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