Phenotypic characterization of Adig null mice suggests roles for adipogenin in the regulation of fat mass accrual and leptin secretion

• Main Authors: Anna Alvarez-Guaita, Satish Patel, Koini Lim, Afreen Haider, Liang Dong, Olivia J. Conway, Marcella K.L. Ma, Davide Chiarugi, Vladimir Saudek, Stephen O’Rahilly, David B. Savage
• Format: Article
• Language: English
• Published: Elsevier 2021-03-01
• Series: Cell Reports
• Subjects: adipogenin; adipose tissue; adipogenesis; leptin; knockout mouse
• Online Access: http://www.sciencedirect.com/science/article/pii/S2211124721001248

Summary: Adipogenin (Adig) is an adipocyte-enriched transmembrane protein. Its expression is induced during adipogenesis in rodent cells, and a recent genome-wide association study associated body mass index (BMI)-adjusted leptin levels with the ADIG locus. In order to begin to understand the biological function of Adig, we studied adipogenesis in Adig-deficient cultured adipocytes and phenotyped Adig null (Adig−/−) mice. Data from Adig-deficient cells suggest that Adig is required for adipogenesis. In vivo, Adig−/− mice are leaner than wild-type mice when fed a high-fat diet and when crossed with Ob/Ob hyperphagic mice. In addition to the impact on fat mass accrual, Adig deficiency also reduces fat-mass-adjusted plasma leptin levels and impairs leptin secretion from adipose explants, suggesting an additional impact on the regulation of leptin secretion.