3D organoids derived from the small intestine: An emerging tool for drug transport research

3D organoids derived from the small intestine: An emerging tool for drug transport research

Small intestine in vitro models play a crucial role in drug transport research. Although conventional 2D cell culture models, such as Caco-2 monolayer, possess many advantages, they should be interpreted with caution because they have relatively poor physiologically reproducible phenotypes and funct...

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Main Authors: Yuanjin Zhang, Shengbo Huang, Weiguo Zhong, Wenxia Chen, Bingyi Yao, Xin Wang
Format: Article
Language:English
Published: Elsevier 2021-07-01
Series:Acta Pharmaceutica Sinica B
Subjects:
3D organoid
Small intestine
Drug transporter
Caco-2 cell monolayer
P-glycoprotein
Online Access:http://www.sciencedirect.com/science/article/pii/S2211383520308340
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spelling doaj-429e37bc39eb4fc89578102fea429d122021-07-25T04:42:59ZengElsevierActa Pharmaceutica Sinica B2211-38352021-07-01117169717073D organoids derived from the small intestine: An emerging tool for drug transport researchYuanjin Zhang0Shengbo Huang1Weiguo Zhong2Wenxia Chen3Bingyi Yao4Xin Wang5Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200051, China; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, ChinaChangning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200051, China; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, ChinaChangning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200051, ChinaChangning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200051, ChinaChangning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200051, ChinaChangning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200051, China; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, China; Corresponding author. Tel.: +86 21 2420 6564; fax: +86 21 5434 4922.Small intestine in vitro models play a crucial role in drug transport research. Although conventional 2D cell culture models, such as Caco-2 monolayer, possess many advantages, they should be interpreted with caution because they have relatively poor physiologically reproducible phenotypes and functions. With the development of 3D culture technology, pluripotent stem cells (PSCs) and adult somatic stem cells (ASCs) show remarkable self-organization characteristics, which leads to the development of intestinal organoids. Based on previous studies, this paper reviews the application of intestinal 3D organoids in drug transport mediated by P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance protein 2 (MRP2). The advantages and limitations of this model are also discussed. Although there are still many challenges, intestinal 3D organoid model has the potential to be an excellent tool for drug transport research.http://www.sciencedirect.com/science/article/pii/S22113835203083403D organoidSmall intestineDrug transporterCaco-2 cell monolayerP-glycoprotein
collection DOAJ
language English
format Article
sources DOAJ
author Yuanjin Zhang
Shengbo Huang
Weiguo Zhong
Wenxia Chen
Bingyi Yao
Xin Wang
spellingShingle Yuanjin Zhang
Shengbo Huang
Weiguo Zhong
Wenxia Chen
Bingyi Yao
Xin Wang
3D organoids derived from the small intestine: An emerging tool for drug transport research
Acta Pharmaceutica Sinica B
3D organoid
Small intestine
Drug transporter
Caco-2 cell monolayer
P-glycoprotein
author_facet Yuanjin Zhang
Shengbo Huang
Weiguo Zhong
Wenxia Chen
Bingyi Yao
Xin Wang
author_sort Yuanjin Zhang
title 3D organoids derived from the small intestine: An emerging tool for drug transport research
title_short 3D organoids derived from the small intestine: An emerging tool for drug transport research
title_full 3D organoids derived from the small intestine: An emerging tool for drug transport research
title_fullStr 3D organoids derived from the small intestine: An emerging tool for drug transport research
title_full_unstemmed 3D organoids derived from the small intestine: An emerging tool for drug transport research
title_sort 3d organoids derived from the small intestine: an emerging tool for drug transport research
publisher Elsevier
series Acta Pharmaceutica Sinica B
issn 2211-3835
publishDate 2021-07-01
description Small intestine in vitro models play a crucial role in drug transport research. Although conventional 2D cell culture models, such as Caco-2 monolayer, possess many advantages, they should be interpreted with caution because they have relatively poor physiologically reproducible phenotypes and functions. With the development of 3D culture technology, pluripotent stem cells (PSCs) and adult somatic stem cells (ASCs) show remarkable self-organization characteristics, which leads to the development of intestinal organoids. Based on previous studies, this paper reviews the application of intestinal 3D organoids in drug transport mediated by P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance protein 2 (MRP2). The advantages and limitations of this model are also discussed. Although there are still many challenges, intestinal 3D organoid model has the potential to be an excellent tool for drug transport research.
topic 3D organoid
Small intestine
Drug transporter
Caco-2 cell monolayer
P-glycoprotein
url http://www.sciencedirect.com/science/article/pii/S2211383520308340
work_keys_str_mv AT yuanjinzhang 3dorganoidsderivedfromthesmallintestineanemergingtoolfordrugtransportresearch
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